球等鞭金藻的异养培养  

Heterotrophic Culture of Isochrysis galbana

作　　者：姬恒 姜爱莉[1] 郭效辰 蒋琴 孟欣 刘宇 管瑞芹 JI Heng;JIANG Ai-li;GUO Xiao-chen;JIANG qin;MENG Xin;LIU yu;GUAN Rui-qin(School of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《广东海洋大学学报》2021年第2期47-52,共6页Journal of Guangdong Ocean University

基　　金：微藻的高密度异养培养(YDZD2028);烟台大学研究生科技创新基金;刺参养殖池塘丝状绿藻暴发过程与细菌群落动态变化的关系及其生态耦合机制研究(41906098)国家自然科学基金青年基金。

摘　　要：【目的】探索球等鞭金藻的异养培养条件。【方法】以球等鞭金藻3011为研究对象,对其进行避光异养培养,采用单因素实验,找出适合其异养生长的碳源、氮源及磷源。【结果与结论】葡萄糖、尿素和磷酸二氢钾分别为较适于球等鞭金藻异养培养的碳源、氮源和磷源;异养状态下球等鞭金藻生物量较高,在葡萄糖浓度40 g/L、尿素1.0 g/L、KH_(2)PO_(4)13.6 mg/L时微藻培养效果最佳,30℃下培养48 h微藻密度达109 mL^(-1),培养96 h密度超过1010 mL^(-1)。【Objective】To explore the optimal heterotrophic culture conditions of Isochrysis galbana.【Method】Isochrysis galbana Parke 3011 was heterotrophic cultured under light protection,single factor experiment was used to find out suitable carbon,nitrogen and phosphorus sources for heterotrophic species.【Results and Conclusion】Glucose and urea were suitable carbon and nitrogen sources for heterotrophic culture of I.galbana.Isochrysis galbana in a heterotrophic state can reach a higher biomass.When the concentration of glucose,urea and potassium dihydrogen phosphate were 40 g/L,1.0g/L and 13.6mg/L respectively,the microalgae could proliferate quickly,the cell density could reach 109 cells/mL at 48 h and 10^(10) cells/mL at 96 h.

关 键 词：球等鞭金藻 异养培养 碳源 氮源 磷源 

分 类 号：S963.21[农业科学—水产养殖]