大黄素对自身免疫性甲状腺炎小鼠的作用机制研究  

The mechanisms of emodin in the treatment of autoimmune thyroiditis in mice

作　　者：张国平[1] 李攀 胡通海 吴可人[2] Guo-ping Zhang;Pan Li;Tong-Hai Hu;Ke-ren Wu(The Second Department of Surgery,Shengzhou Hospital of Traditional Chinese Medicine,Shengzhou,Zhejiang 312400,China;Department of Hepatobiliary Surgery,The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou,Zhejiang 310000,China)

机构地区：[1]嵊州市中医院外二科,浙江嵊州312400 [2]浙江中医药大学附属第一医院肝胆外科,浙江杭州310000

出　　处：《中国现代医学杂志》2021年第6期32-36,共5页China Journal of Modern Medicine

基　　金：2018年度绍兴市科技计划(医卫类)项目(No:2018C30160)。

摘　　要：目的探讨大黄素治疗自身免疫性甲状腺炎(AT)小鼠的作用机制。方法选择60只小鼠随机分为正常组、模型组和实验组,每组20只。实验组给予75 mg/kg大黄素灌胃,对照组和模型组灌胃等剂量生理盐水,均1次/d,连续8周。采用酶联免疫吸附试验测定甲状腺球蛋白抗体(TGAb)和甲状腺过氧化物酶自身抗体(TPOAb)水平;观察甲状腺淋巴细胞浸润程度;采用流式细胞术测定T淋巴细胞亚群变化。结果正常组、模型组和实验组小鼠血清TGAb和TPOAb水平比较,差异有统计学意义(P<0.05);实验组低于模型组(P<0.05)。3组小鼠淋巴细胞浸润程度比较,差异有统计学意义(P<0.05);实验组低于模型组(P<0.05)。3组小鼠CD3+CD4+和CD3+CD8+比较,差异有统计学意义(P<0.05);实验组低于模型组(P<0.05)。结论大黄素可下调AT小鼠血清TGAb和TPOAb水平,减轻淋巴细胞浸润程度,调节小鼠免疫功能。Objective To investigate the mechanisms of emodin in the treatment of autoimmune thyroiditis(AT)in mice.Methods The 60 mice were randomly divided into normal group,model group and experimental group,with 20 mice in each group.The experimental group was intragastrically given 75 mg/kg emodin once a day for 8 weeks;the control group and the model group were given the same dose of normal saline once a day for 8 weeks.The levels of thyroglobulin antibody(TGAb)and thyroid peroxidase antibody(TPOAb)were measured by enzyme-linked immunosorbent assay;the degree of lymphocyte infiltration in the thyroid gland was observed;and the changes of T-lymphocyte subsets in peripheral blood were measured by flow cytometry.Results The serum TGAb and TPOAb levels were significantly different among the three groups,and those in experimental group were lower than those in model group(P<0.05).The degree of lymphocyte infiltration was distinct among the groups,and that in the experimental group was lower compared to that in model group(P<0.05).Besides,the CD3+CD4+and CD3+CD8+cell counts were different among the groups,and those in the experimental group were lower in comparison to those in model group(P<0.05).Conclusion Emodin can reduce the serum TGAb and TPOAb,ameliorate the degree of lymphocyte infiltration and regulate the immune function in AT mice.

关 键 词：自身免疫性甲状腺炎 大黄素 作用机制 小鼠 

分 类 号：R581[医药卫生—内分泌]