不同基质对茅苍术移栽组培苗叶片导水度及光合的影响  

Effects of Different Substrates on Leaf Water Conductivity and Photosynthesis of Tissue Culture Plantlets of Atractylodes lancea

作　　者：陈璐[1] 邢德科[1] 吴沿友[2] 赵玉国[1] 周荣山 CHEN Lu;XING Deke;WU Yanyou;ZHAO Yuguo;ZHOU Rongshan(Key Laboratory of Modern Agricultural Equipment and Technology,Ministry of Education,Institute of Agricultural Engineering,Jiangsu University,Zhenjiang Jiangsu 212013,China;Research center for Environmental Bio-Science and Technology,State Key Laboratory of EnvironmentalGeochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang Guizhou 550002,China;Jiangsu Golden Age Health Care Development Co.,Ltd,Zhenjiang Jiangsu 212115,China)

机构地区：[1]现代农业装备与技术教育部重点实验室/江苏大学农业工程学院,江苏镇江212013 [2]环境地球化学国家重点实验室环境生物科技研究中心/中国科学院地球化学研究所,贵州贵阳550002 [3]江苏金色年华健康养生发展有限公司,江苏镇江212115

出　　处：《种子》2021年第2期7-12,19,共7页Seed

基　　金：国家重点研发计划项目(2016 YFC 0502602);镇江市丹徒区重点研发计划项目(NY 2018004)。

摘　　要：选取茅苍术(Atractylodes lancea(Thunb.)DC)组培苗为实验材料,进行驯化栽培,研究不同基质的物理性质,比较茅苍术组培苗在各基质中的叶片固有导水度、光合作用及生长状况,以筛选出最优炼苗基质。结果表明,基质持水特性是影响组培苗驯化的关键因素,T 3(蛭石∶珍珠岩∶泥炭土=1∶1∶2)基质中培养的驯化苗的叶片水分状况、光合及生长方面与其他处理相比表现较好,可作为茅苍术组培苗驯化的理想基质。基于生理电阻的叶片固有导水度可用于水分状况的及时诊断。研究结果为快速获得大量优势茅苍术种苗提供指导,同时为组培苗驯化炼苗提供理论基础和技术支撑。In this study,the tissue culture seedlings of Atractylodes lancea(Thunb.)DC were selected as experimental materials for domestication and cultivation.The physical properties of different substrates were studied,and the inherent water conductivity,photosynthesis and growth status of the leaves of tissue culture seedlings of A.lancea were also compared in each substrate,so as to select the optimal substrate.The results showed that the water-holding capacity of substrate was the key factor affecting domestication of tissue culture seedlings.Compared with other treatments,T 3(vermiculite∶perlite∶peat=1∶1∶2)showed better leaf water,photosynthesis and growing status,and could be used as an ideal substrate for domestication of tissue culture seedlings of A.lancea.Leaf intrinsic water conductivity based on physiological resistance could be used for timely diagnosis of water status.The results of this study provided guidance for rapid acquisition of a large number of dominant A.lancea seedlings,and provided theoretical basis and technical support for domestication of tissue culture seedlings.

关 键 词：生理电阻 驯化 固有导水度 光合 组织培养 

分 类 号：Q945.1[生物学—植物学]