依布硒啉对多重耐药金黄色葡萄球菌的杀伤效果研究  

The Antibacterial Effect of Ebselen Against Multidrug-Resistant Staphylococcus Aureus

作　　者：谭超 邹黎黎[1,2] Tan Chao;Zou Lili(College of Biological and Pharmaceutical Science,Hubei Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang,443002,China;Department of Transfusion,Yichang Central People's Hospital,The First College of Clinical Medical Science,China Three Gorges University,Yichang 443003,China)

机构地区：[1]天然产物研究与利用湖北省重点实验室(三峡大学),湖北宜昌443002 [2]三峡大学第一临床医学院[宜昌市中心人民医院]输血科,湖北宜昌443003

出　　处：《巴楚医学》2021年第1期65-68,85,共5页Bachu Medical Journal

基　　金：湖北省教育厅基金(No:B2016022);湖北省自然科学基金(No:2014CFB307);天然产物研究与利用湖北省重点实验室(三峡大学)开放课题(No:NPRD-2018009);宜昌市医疗卫生计划项目(No:A19-301-15)。

摘　　要：目的:研究抗卒中老药依布硒啉(Ebselen)对临床分离多重耐药性金黄色葡萄球菌的杀伤效果,并探究其作用靶点。方法:收集我院多重耐药金黄色葡萄球菌共计108株,通过微孔及涂布法观察Ebselen对金黄色葡萄球菌的杀菌效力;通过5,5’-二硫代双(2-硝基苯甲酸)(DTNB)实验检测Ebselen对金黄色葡萄球菌硫氧还蛋白还原酶(TrxR)活性的影响。结果:Ebselen可高效杀伤分离得到的108株多重耐药金黄色葡萄球菌,最小抑菌浓度(MIC)5~40μM;且Ebselen作用后的细菌TrxR活性降低至8.3%~51.9%(对照组为100%)。结论:Ebselen可通过降低TrxR的活性,实现对多重耐药性金黄色葡萄球菌的高效杀伤。Objective:To investigate the antibacterial effect of Ebselen an old anti-apoplexy drug on clinical isolation of multi-drug resistant(MDR)staphylococcus aureus and explore its target.Methods:There were 108 strains of MDR staphylococcus aureus collected in our hospital.The bactericidal effects of Ebselen on these strains were evaluated using microporous and coating methods.The effect of Ebselen on the thioredoxin reductase(TrxR)activity in staphylococcus aureus was determined by[5,5'-Dithiobis-(2-nitrobenzoic acid)](DTNB)assay.Results:Ebselen effectively killed the 108 strains of MDR staphylococcus aureus,with the minimum inhibitory concentration(MIC)between 5μM and 40μM.Ebselen significantly reduced the intracellular TrxR activity to 8.3%~51.9%(100%in the control group).Conclusion:Ebselen could effectively kill MDR staphylococcus aureus by inhibition of TrxR activity.

关 键 词：依布硒啉 多重耐药性金黄色葡萄球菌 硫氧还蛋白还原酶 

分 类 号：R446.5[医药卫生—诊断学]