干旱复水对5种马尾松菌根化苗木生理特性的影响  

Effects of drought and rewatering on physiological properties of five species of Pinus massoniana seedlings

作　　者：王艺 涂桂英[2] WANG Yi;TU Guiying(College of Ecological Environment and Engineering,Guizhou Minzu University,Guiyang 550025,Guizhou,China;College of Forestry,Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州民族大学生态环境工程学院,贵州贵阳550025 [2]贵州大学林学院,贵州贵阳550025

出　　处：《中南林业科技大学学报》2021年第4期20-30,46,共12页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31400537);贵州省教育厅青年科技人才成长项目(黔教合KY字〔2018〕134);贵州民族大学自然科学基金(GZMU〔2019〕YB18)。

摘　　要：【目的】探讨持续干旱及复水后外生菌根真菌对马尾松幼苗生长及生理特性的影响。【方法】以马尾松幼苗为材料,开展接种褐环乳牛肝菌1(S1)、褐环乳牛肝菌7(S7)、鸡油菌(G)、彩色豆马勃(C)和土生空团菌(T)5种菌种的盆栽试验,未接种苗木作对照(CK),在温室内进行人工模拟自然干旱。【结果】1)持续干旱及复水过程中,在接种5种外生菌根真菌作用下,苗木苗高、地径、生物量和根冠比显著提高(P<0.05),其中接种S1效果最佳。2)持续干旱及复水后,外生菌根真菌显著提高苗木含水量,降低苗木水分饱和亏缺,提高苗木保水力(P<0.05),但适度胁迫有利于植物水分的补偿作用。3)持续干旱胁迫下,苗木可溶性糖含量在干旱胁迫第14天达最大,接种S1的含量最高,提高了33.1%,但菌根化苗木与对照间差异不显著(P>0.05);菌根化苗木显著提高了可溶性蛋白含量,增幅最大达109%,降低了脯氨酸含量,降幅最大达66.5%(P<0.05);复水后苗木可溶性糖、脯氨酸含量恢复程度与胁迫时间有关,菌根化苗木可溶性蛋白含量恢复能力大于对照,差异显著(P<0.05)。【结论】持续干旱及复水后外生菌根能够促进马尾松苗木生长,促进其根系形态形成,改善水分利用状况和渗透调节物质含量,其中S1的苗木抗旱能力最强,而根系形态是影响马尾松不同苗木抗旱性的关键指标。【Objective】To explore the effects of ectomycorrhizal fungi on the growth and physiological characteristics of Pinus massoniana seedlings after continuous drought and rewatering.【Method】To carry out the Pinus massoniana vaccination ring in Suillus luteus(S1&S7),Cantharellus cibarius(G),Pisolithus tinctorius(C)and Cenococcum geophilum(T)of pot experiment,unvaccinated seedlings as control(CK),the artificial simulation natural drought in the greenhouse.【Result】1)In the process of continuous drought and rewatering,the five ectomycorrhizal fungi inoculated all significantly increased the seedling height,ground diameter,biomass and root-cap ratio(P<0.05),among which S1 inoculated had the best effect.2)After continuous drought and rewatering,ectomycorrhizal fungi can significantly increase the water content of seedlings,reduce the water saturation deficit of seedlings,and improve the water retention of seedlings(P<0.05).However,moderate stress is beneficial to the compensation of plant water.3)Under continuous drought stress,the soluble sugar content of seedlings reached the maximum on the 14 d of drought stress,and the content of inoculation S1 was the highest,with an increase of 33.1%.However,there was no significant difference between mycorrhizal seedlings and the control(P>0.05).Mycorrhizated seedlings significantly increased the soluble protein content by up to 109%and reduced the proline content by up to 66.5%(P<0.05).The degree of recovery of soluble sugar and proline content in seedlings after rewatering was related to the stress time,and the ability of recovery of soluble protein content in mycorrhizated seedlings was greater than that in the control group,with significant difference(P<0.05).【Conclusion】After continuous drought and rewatering,ectomycorrhizal seedlings could promote the growth of Pinus massoniana seedlings,promote the root morphogenesis,improve the water use status and osmotic regulation substance content,among which Suillus luteus1 seedlings had the strongest drought resistance a

关 键 词：马尾松 外生菌根真菌 干旱胁迫 生长形态 生理特性 

分 类 号：S718.8[农业科学—林学]