基于物体认知法研究慢性睡眠干扰对小鼠学习记忆的影响  

Study on Learning and Memory Impairment in Mice Induced by Chronic Sleep Interruption Based on Object Recognition Task

作　　者：李玉姣 王海霞 陈善广[4] 刘新民 李莹辉[5] 曲丽娜[5] 王琼[1,2,6] Li Yujiao;Wang Haixia;Chen Shanguang;Liu Xinmin;Li Yinghui;Qu Lina(不详;Sino-Portugal TCM International Coop-eration Center,Affiliated TCM Hospital,Southwest Medical University,Luzhou Sichuan 646000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]西南医科大学附属中医医院中葡中医药国际合作中心,四川泸州646000 [3]中国医学科学院北京协和医学院药用植物研究所药理毒理研究中心,北京100193 [4]中国航天员科研训练中心人因工程重点实验室,北京100094 [5]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094 [6]中国农业科学院农产品加工研究所,北京100193

出　　处：《航天医学与医学工程》2021年第1期25-30,共6页Space Medicine & Medical Engineering

基　　金：四川省国际科技创新合作/港澳台科技创新合作项目(2019YFH0023);航天医学基础与应用国家重点实验室开放基金资助项目(SMFA18K06)。

摘　　要：目的基于不同物体认知实验模式开展慢性睡眠干扰引起的学习记忆功能研究,为航天医学相关药物筛选提供行为学检测方法参考。方法48只雄性小鼠随机分为3批,每批对照组和模型组各8只;模型组小鼠采用滚筒法造模模拟睡眠干扰,滚筒设置参数为:60 s/圈,转1圈,间隔2 min;连续造模14天后分别对3批动物进行时序记忆实验、情景记忆实验以及新物体识别实验,以检测小鼠学习记忆能力。结果在时序记忆和情景记忆实验中,与对照组相比,模型组小鼠的相对辨别指数显著降低(P<0.05,P<0.01)。在新物体识别实验中,模型组小鼠相对辨别指数在不同时间段均降低(P<0.05,P<0.01)。结论慢性睡眠干扰可损害小鼠的物体认知时序记忆、情景记忆和新物体识别能力。Objective The learning and memory dysfunction induced by chronic sleep interruption was studied with different object recognition tests to provide a behavioral test method for screening space medicine related drugs.Methods Forty-eight male mice were randomly divided into three batches and each batch were randomly divided into the control group and the model group with eight animals in each group.Mice in the model group were treated with rotating sleep interferometer for 14 days(The rotation speed was 60 s one rotation and there was a 2 min pause after each rotation).Then the temporal order memory task,the object context recognition task,and the novel object recognition task were tested for the three batches of mice respectively to assess the recognition behavioral activities of mice.Results For the temporal order memory task and object context recognition task,there was a significant decrease in discrimination index in the model group as compared with the control group(P<0.05,P<0.01).For the novel object recognition task,the discrimination index significantly decreased in the model group at different time phases(P <0.05,P<0.01).Conclusion Chronic sleep interruption could impair the object recognition memory,the temporal memory and object context memory in mice.

关 键 词：慢性睡眠干扰 时序记忆实验 情景记忆实验 新物体识别实验 航天飞行 

分 类 号：R857.1[医药卫生—航空、航天与航海医学]