Transcriptional control of chicken KLF7 promoter in preadipocytes  

作　　者：Tao Lin Yuechan Chen Yanling Zhang Yaoyao Li Lingyu Gao Zhiwei Zhang 

机构地区：[1]School of Medicine,Shihezi University,Shihezi 832000,China [2]First Affiliated Hospital of School of Medicine,Shihezi University,Shihezi 832000,China [3]Pharmaceutical Department,People’s Hospital of Anyang,Anyang 455000,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第2期149-159,共11页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(No.31960647 and 31501947);the Youth Innovative Talents Project of Shihezi University(No.CXBJ201905).

摘　　要：Krüppel-like factor 7 (KLF7) has been reported to inhibit adipogenesis and regulate the development of the nervous system. However, transcription regulation of KLF7 remains poorly understood. In the current study, a 2196-bp-long 5′-flanking sequence of chicken KLF7 (−2286 bp to −91 bp, upstream of the translation start site) was studied for promoter activity, and there was a remarkable promoter activity in this sequence (P<0.05). The 5′-truncated mutation analysis showed that a minimal promoter was on the sequence from −241 bp to −91 bp. In addition, GATA2 overexpression facilitated the promoter activity of pGL3-KLF7(−2286/−91), pGL3-KLF7(−1215/−91), pGL3-KLF7(−521/−91), and pGL3-KLF7(−241/−91), and GATA3 overexpression inhibited the promoter activity of pGL3-KLF7(−1845/−91), pGL3-KLF7(−1215/−91), pGL3-KLF7(−521/−91), and pGL3-KLF7(−241/−91) in chicken preadipocytes (P<0.05). Knockdown of GATA2 expression inhibited the promoter activity of pGL3-KLF7(−1215/−91) and pGL3-KLF7(−241/−91), and knockdown of GATA3 expression facilitated the promoter activity of pGL3-KLF7(−521/−91) and pGL3-KLF7(−241/−91) (P<0.05). Additionally, overexpression and knockdown analyses showed that GATA3 inhibited KLF7 mRNA expression (P<0.05), and both overexpression and knockdown of GATA2 resulted in the downregulation of KLF7 mRNA expression in chicken preadipocytes (P<0.05). Western blot analysis in chicken preadipocytes showed that GATA2 facilitated KLF7 expression and GATA3 inhibited KLF7 expression. Mutation analysis showed that the motif of ‘GGATCTATCA’ (−107 bp/−98 bp) might be a cis-regulation element, which is involved in the KLF7 expression regulation by GATA3 in chicken preadipocytes. These results provided some details of KLF7 transcription regulation in chicken adipose tissue.

关 键 词：CHICKEN PREADIPOCYTES Kr¨uppel-like factor 7(KLF7) transcription regulation PROMOTER 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]