A novel polyethyleneimine-decorated FeOOH nanoparticle for efficient siRNA delivery  

作　　者：Shuai Guo Bei Liu Mengjie Zhang Chunhui Li Xiaoxia Wang Yuhua Weng Lele Li Yuanyu Huang 

机构地区：[1]School of Life Science,Advanced Research Institute of Multidisciplinary Science,Key Laboratory of Molecular Medicine and Biotherapy,Institute of Engineering Medicine,Beijing Institute of Technology,Beijing 100081,China [2]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology(NCNST),Beijing 100190,China [3]School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China [4]College of Materials Science and Optoelectronic Technology,University of Chinese Academy of Sciences,Beijing 100049,China [5]Hunan University of Science and Engineering,Yongzhou 425199,China [6]Institute of Molecular Medicine,Peking University,Beijing 100871,China

出　　处：《Chinese Chemical Letters》2021年第1期102-106,共5页中国化学快报（英文版）

基　　金：supported by the Hunan Provincial Natural Science Foundation of China(Nos.2018JJ1019,2019JJ50196);the Hu-Xiang Young Talent Program(No.2018RS3094);the National Natural Science Foundation of China(Nos.31871003,31901053);the Beijing Institute of Technology Research Fund Program for Young Scholars and the Fundamental Research Funds for the Central Universities(No.2018CX01023);the Beijing-Tianjin-Hebei Basic Research Cooperation Project(No.19JCZDJC64100);the Beijing Nova Program from Beijing Municipal Science&Technology Commission(No.Z201100006820005);the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology(2020-2022)。

摘　　要：Despite the promising prospect of small interfering RNA(siRNA) for the treatment of diverse diseases,it remains challenging to develop novel delive ry materials to desired tissues and cells.In this study,a novel iron oxyhydroxide(FeOOH) nanoparticle(NP) whose surface was modified with branched polyetherimide(PEI) was developed to deliver siRNA into the cancer cells.It was demonstrated that PEI-FeOOH(PFeOOH) efficiently complexed siRNA,mediated effective cellular uptake and endosomal escape,thereby triggering robust gene silencing in vitro.In addition,PFeOOH/siRNA formulation loading with anti-RRM2 siRNA effectively inhibited the growth of tumor tissues,and exhibited excellent safety profiles in vivo.Therefore,this study conceptually provided a FeOOH-based nucleic acid delivery vesicle which can potentially use to achieve diagnosis and therapy simultaneously.

关 键 词：FEOOH siRNA delivery RRM2 RNA interference POLYETHERIMIDE Cancer treatment 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R450[医药卫生—治疗学]