Pressure controllable aptamers picking strategy by targets competition  

作　　者：Ge Yang Chao Zhu Liping Zhao Linsen Li Yuanyu Huang Yukui Zhang Feng Qu 

机构地区：[1]Key Laboratory of Molecular Medicine and Biotherapy,School of Life Science,Beijing Institute of Technology,Beijing 100081,China [2]Advanced Research Institute of Multidisciplinary Science,Beijing Institute of Technology,Beijing 100081,China [3]National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics,Chinese Academy of Science,Dalian 116011,China

出　　处：《Chinese Chemical Letters》2021年第1期218-220,共3页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.21675012,21874010 and 21827810);the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology。

摘　　要：Selection of aptamers with high affinity and good specificity requires multiple rounds of alternating steps of separation and PCR amplification.Herein,we proposed a novel high-efficiency aptamers picking strategy:One-round pressure controllable selection(OPCS).OPCS integrates four types of screening superiority,high-efficiency separation,one-round selection and PCR amplification,synchronous negative selection and targets competition.The controllable screening pressure can be achieved through two approaches,balanced competition by the regulation of protein concentration,and dominant competition by introducing a predatory protein with high concentration.In OPCS process,two proteins were co-incubated with one ssDNA library,and each protein bound its favorable sequences specifically and formed protein-ss D NA complex re spectively.Meanwhile,one protein could supply/sufferthe picking pressure of affinity and specificity to/from another,which eliminated weakly bound or unbound sequences for each other.Two complexes could be separated and collected conveniently,and aptamers for two proteins obtained synchronously with high affinity and good specificity.This strategy not only provides a more effective way for aptamers selection,but shows great potential in other ligands or drugs selection.

关 键 词：Capillary electrophoresis Aptamer selection Screening pressure controllable Targets-synchronous competition One-round selection 

分 类 号：Q503[生物学—生物化学]