Ultra-small nanodots coated with oligopeptides providing highly negative charges to enhance osteogenic differentiation of hBMSCs better than osteogenic induction medium  

作　　者：Yueqi Zhao Lin Meng Kai Zhang Yuanqing Sun Yue Zhao Zhe Yang Yangliu Lin Xinrui Liu Hongchen Sun Bai Yang Quan Lin 

机构地区：[1]State Key Laboratory of Supramolecular Structure and Material,College of Chemistry,Jilin University,Changchun 130012,China [2]School and Hospital of Stomatology,China Medical University,Shenyang 110002,China [3]State Key Laboratory of Heavy Oil Processing,College of Science,China University of Petroleum,Beijing 102249,China [4]School and Hospital of Stomatology,Jilin University,Changchun 130021,China [5]Department of Neurosurgical Oncology,The First Hospital of Jilin University,Changchun 130021,China

出　　处：《Chinese Chemical Letters》2021年第1期266-270,共5页中国化学快报（英文版）

基　　金：supported by the National Nature Science Foundation of China(Nos.51861145311,22005338);Science Foundation of China University of Petroleum,Beijing(No.2462017YJRC027);Open Project of State Key Laboratory of Superhard Materials(Jilin University 201802)。

摘　　要：For bone regenerative engineering,it is a promising method to form skeletal tissues differentiating from human bone morrow mesenchyme stem cells(hBMSCs).However,it is still a critical challenge to efficiently control ostogenesis and clearly reveal the influence factor.To this end,the fluorescent gold nanodots(Au NDs) with highly negative charges as osteogenic induction reagent are successfully synthesized,which display better than commercial osteogenic induction medium through the investigations of ALP activity(2.5 folds) and cytoskeleton staining(1.5 folds).Two kinds of oligopeptides with different bio-structures(cysteine,Cys and glutathione,GSH) are selected for providing surficial charges on Au NDs.It is revealed that Au-Cys with more negative charges(-51 mV) play better role than Au-GSH(-19 mV) in osteogenic differentiation,when both of them have same size(~2 nm),sphere shape and show similar cell uptake amount.To explore deeply,osteogenesis related signaling pathways are monitored,revealing that the enhancement of osteogenic differentiation was through autophagy signaling pathway triggered by Au-Cys.And the promotion of highly negative charges in osteogenic diffe rentiation was further proved via sliver nanodots(Ag NDs,Ag-Cys and Ag-GSH) and carbon nanodots(CDs,Cys-CDs and GSH-CDs).This work indicates part of insights during hBMSCs differentiation and provides a novel strategy in osteogenic differentiation process.

关 键 词：Osteogenic differentiation Negative charges from oligopeptide Sub-3 nm fluorescent Au nanodots Better than osteogenic induction medium Autophagy 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学]