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作 者:马婕 李开枝[2] 邱大俊[2] 谭烨辉[2] 黄良民[2,3] 张俊彬 MA Jie;LI Kaizhi;QIU Dajun;TAN Yehui;HUANG Liangmin;ZHANG Junbin(National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;University of Chinese Academy of Science,Beijing 100049,China;Shenzhen Key Laboratory of Marine Bioresource&Eco-environmental Science,College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518000,China)
机构地区:[1]国家实验渔业科学教育示范中心,农业部水产遗传资源开发利用重点实验室,上海海洋大学上海水产养殖工程研究中心,上海201306 [2]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广州510301 [3]中国科学院大学,北京100049 [4]深圳大学生命科学与海洋学院,深圳市海洋生物资源与生态环境科学重点实验室,深圳518000
出 处:《生态科学》2021年第2期9-17,共9页Ecological Science
基 金:国家973项目(2015CB452904);国家自然科学基金重点项目(41130855)。
摘 要:采用Illuminate高通量测序技术分析了大亚湾肥胖软箭虫(Flaccisagitta enflata)的食性。在夏季(2017年8月)和冬季(2018年1月)对大亚湾内不同站位拖网采获的四组样品中,分别挑取毛颚类优势种肥胖软箭虫进行18S rDNA V4区扩增;通过高通量测序得到四组样品的序列,经过处理每组样品得到约30,000条高质量序列。分析结果表明,大亚湾肥胖软箭虫的食物来源于16个门类生物,主要的优势类群分别来自于3个门类的浮游动物(刺胞动物门46.16%、节肢动物门的桡足类19.16%和栉水母动物门14.22%),真菌类的2个门类(子囊菌门14.04%和担子菌亚门4.48%),以及少量浮游植物类群的2个门(褐藻门0.06%和隐藻门0.03%)。另外,还检测出少量肥胖软箭虫可能摄食的纤毛虫、线虫、住囊虫、海葵等。夏季湾内肥胖软箭虫食物中子囊菌门贡献最高(43.3%),这与大亚湾海水养殖等人类活动影响有关;夏季湾口外海水带来丰富的暖水性栉水母,占肥胖软箭虫总食物丰度的55.25%。冬季湾内肥胖软箭虫摄食哲水蚤比例达49.94%,而湾口肉质介穗水母占比高达85.3%。分析结果可见,肥胖软箭虫的摄食具有偏好性,且明显存在季节和区域差异。研究探讨了大亚湾不同环境下肥胖软箭虫食性转变及其食物来源的季节和区域差异,为深入揭示浮游动物在海湾生态系统物质与能量传递过程的作用机制提供了重要基础资料。In this paper,the diet of Flaccisagitta enflata in Daya Bay was analyzed using Illuminate High-throughput Sequencing Technology.In summer(August 2017)and winter(January 2018),the four groups of samples taken from different trawls in Daya Bay were picked up for the 18S rDNA V4 region.The sequences of the four sets of samples were obtained by high-throughput sequencing,and about 30000 high-quality sequences were obtained after processing each set of samples.The results showed that the food of Flaccisagitta enflata in Daya Bay originated from 16 phyla.The main dominant groups were from the three phyla of zooplankton group(46.16%cnidaria,19.16%copepod,14.22%ctenophore);2 phyla of fungi(14.04%ascomycetes,4.48%basidiomycosis);and 2 phyla of a little of phytoplankton groups(0.06%stramenopiles and 0.03%cryptophyta).In addition,a small number of ciliates,nematodes,cysticercosis,and anemones that might be ingested by Flaccisagitta enflata were also detected.In the summer,the weight of the neutropha in the diet of the Flaccisagitta enflata was the highest(43.3%)within the bay,which was related to the impact of human activities such as marine aquaculture in Daya Bay.The seawater outside the bay was rich in warm water and jellyfish,which accounted for the total food of Flaccisagitta enflata 55.25%of the degree.In the winter,the proportion of Flaccisagitta enflata feeding on Calanus was 49.94%,while in winter,the proportion of Hydractinia in the bay mouth was as high as 85.3%.The results reveal the changes in the feeding habits of Flaccisagitta enflata in different environments,highlighting the important role of Flaccisagitta enflata in the feeding process and ecosystem,and will help to understand the important role of zooplankton in the process of matter and energy transfer.
关 键 词:肥胖软箭虫 大亚湾 高通量测序 18S rDNA 食性
分 类 号:SQ178.1
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