X射线照射对人脐静脉内皮细胞焦亡的影响  

Induction of pyroptosis by X-rays in human umbilical vein endothelial cells

作　　者：李辰[1] 田梅[1] 齐雪松[1] 王春燕[1] 曲功霖[1] 邵帅[1] 王成芳 苟巧[1] LI Chen;TIAN Mei;QI Xuesong;WANG Chunyan;QU Gonglin;SHAO Shuai;WANG Chengfang;GOU Qiao(National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Key Laboratory of Radiological Protection and Nuclear Emergency,China CDC,Beijing 100088,China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《癌变．畸变．突变》2021年第2期89-94,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：中国疾控中心辐射安全所青年科学研究所长基金(2020-07)。

摘　　要：目的:探讨X射线照射对人脐静脉内皮细胞(HUVEC)焦亡的影响。方法:单次10 Gy X射线照射HUVEC细胞,采用ELISA法检测照射后0~72 h HUVEC分泌细胞因子IL-1β、IL-6、TNF-α和IL-18的浓度,Western blot法检测照射后6~48 h HUVEC内Caspase-1的活化水平,采用透射电子显微镜检测照射后72 h HUVEC形态的变化。采用流式细胞术检测单次10 Gy以及多次小剂量(2.5 Gy/d,连续4 d)照射后HUVEC焦亡率的变化。结果:10 Gy X射线照射后0~72 h HUVEC中IL-1β、IL-6、TNF-α和IL-18浓度与对照组(0 Gy组)相比均明显升高(P<0.05)。10 Gy X射线照射后6~48 h细胞内Caspase-1活化水平升高(P<0.05),细胞呈现体积增大肿胀状态。单次10 Gy照射组和多次小剂量照射组细胞焦亡率均明显高于空白对照组(P<0.01);此外,单次10 Gy照射组细胞焦亡率较多次小剂量照射组细胞升高约1倍(P<0.01)。结论:X射线照射可引起人脐静脉内皮细胞HUVEC焦亡;在总剂量相同情况下,单次大剂量照射引起的HUVEC焦亡水平明显高于多次小剂量照射。OBJECTIVE:To investigate the effects of X-ray irradiation on pyroptosis of human umbilical vein endothelial cells(HUVEC).METHODS:Pyroptosis is an inflammatory programmed cell death process.Cells were irradiated with a single dose of 10 Gy X-ray.In order to investigate the occurrence of X-ray induced pyroptosis,levels of IL-1β,IL-6,TNF-αand IL-18 which were secreted by HUVEC cells from 0 to 72 h after the radiation were determined using the ELISA assay.Caspase-1 which is cleaved to the activated state is a key step of pyroptosis.Therefore,the activated levels of Caspase-1 were detected using Western blot at 6-48 h after the single dose of 10 Gy X-ray irradiation.Morphological changes of HUVECs were detected using transmission electron microscopy(TEM)at 72 h after the irradiation.Flow cytometry(FCM)was used to detect the changes of pyroptosis after a single dose and a small dose of multiple exposures(2.5 Gy/d×4 d).RESULTS:Compared with the control group,the levels of IL-1β,IL-6,TNF-αand IL-18 in HUVEC cells 0-72 h after the radiation were significantly higher(P<0.05)and the levels of activated Caspase1 at 6-48 h after the radiation was increased(P<0.05).The cell volumes were increased with swelling.The percentages of pyroptoti cells after the single and multiple exposures were significantly higher than that of the control group(P<0.01).In addition,the pyroptotic death rates after the single dose was twice as high as that after the multiple doses(P<0.01).CONCLUSION:X-ray exposure induced pyroptosis in HUVEC cells.Under the same total dose,the damage levels from the single dose exposure were significantly higher than that from the multiple exposures.

关 键 词：X射线 人脐静脉内皮细胞 细胞焦亡 半胱氨酸蛋白酶-1 

分 类 号：R994.6[医药卫生—毒理学]