miR-134及miR-301在CHC的表达及与肝功能、病毒载量的相关性  

Expression of miR-134 and miR-301 in CHC and the correlations with liver function and viral load

作　　者：彭燕[1] 尚雪飞[1] 孙鹏娟[1] 王楷翔 Peng Yan;Shang Xuefei;Sun pengjuan;Wang Kaixiang(Department of Pathology,Baoding First Hospital,Baoding 071000,China;Department of Clinical Laboratory,Baoding People’s Hospital,Baoding 071000,China)

机构地区：[1]保定市第一医院病理科,071000 [2]保定市人民医院分子生物学实验室,071000

出　　处：《国际病毒学杂志》2021年第1期37-41,共5页International Journal of Virology

基　　金：保定市科学计划与发展指导计划项目(16ZF052)。

摘　　要：目的:探讨慢性丙型肝炎(chronic hepatitis C,CHC)患者血清miR-134及miR-301表达水平与丙氨酸氨基转移酶(alanine aminotransferase,ALT)和丙肝病毒载量(hepatitis C virus-RNA,HCV-RNA)的相关性。方法:选取我院收治的125例CHC患者作为CHC组和健康体检正常者60例作为对照组。CHC患者根据HCV-RNA的检测结果,分为低病毒载量组、中病毒载量组和高病毒载量组。根据ALT的检测结果,ALT正常组、ALT轻度升高组和ALT明显升高组。检测各组血清miR-134及miR-301表达水平和HCV-RNA含量,分析miR-134、miR-301表达水平与ALT、HCV-RNA的相关性。结果:CHC组血清miR-134及miR-301表达水平均明显高于对照组(t=12.874,P<0.01;t=12.305,P<0.01)。ALT明显升高组血清miR-134及miR-301表达水平明显高于轻度升高组和正常组(P<0.01)。高病毒载量组血清miR-134及miR-301表达水平明显高于中病毒载量组和低病毒载量组(P<0.01),且中病毒载量组血清miR-134及miR-301表达水平明显高于低病毒载量组(P<0.01)。相关分析显示,血清miR-134及miR-301表达水平与ALT、HCV-RNA均呈正相关(r=0.748,P<0.01;r=0.825,P<0.01;r=0.713,P<0.01;r=0.796,P<0.01)。结论:CHC患者血清miR-134及miR-301表达水平明显升高,与ALT、HCV-RNA均呈正相关,提示miR-134及miR-301可能参与CHC发生发展。Objective To investigate the relationships between the expressions of serum miR-134 and miR-301 and levels of alanine aminotransferase(ALT)and hepatitis C virus RNA(HCV-RNA)among patients with chronic hepatitis C(CHC).Methods The 125 CHC patients admitted to our hospital were selected as the CHC group and 60 healthy people as the control group.CHC patients were divided into low viral load group,medium viral load group and high viral load group according to the results of HCV-RNA.The patients were also divided into ALT normal group,ALT slightly elevated group and ALT significantly increased group according to the ALT results.The expression levels of serum miR-134 and miR-301 and HCV-RNA level in each group were detected,and the correlations between the expression levels of miR-134,miR-301 and levels of ALT and HCV-RNA were analyzed.Results The levels of miR-134 and miR-301 in CHC group were significantly higher than those in the control group(t=12.874,P<0.01;t=12.305,P<0.01),respectively.The expression levels of serum miR-134 and miR-301 in the ALT significantly increased group were significantly higher than those in ALT slightly elevated group and ALT normal groups(P<0.001),respectively.The expression levels of serum miR-134 and miR-301 in the high viral load group were significantly higher than those in the middle viral load group and the low viral load group(P<0.001),respectively and the expression levels in the middle viral load group were significantly higher than those in the low viral load group(P<0.001).The correlation analysis showed that the expression levels of serum miR-134 and miR-301 were positively correlated with levels of ALT and HCV-RNA(r=0.748,P<0.01;r=0.825,P<0.01;r=0.713,P<0.01;r=0.796,P<0.01),respectively.Conclusions The expression levels of serum miR-134 and miR-301 in CHC patients increased significantly and positively correlated to levels of ALT and HCV-RNA,suggesting that miR-134 and miR-301 may be involved in the development of CHC.

关 键 词：慢性丙型病毒性肝炎 miR-134 miR-301 病毒载量 丙氨酸氨基转移酶 

分 类 号：R512.63[医药卫生—内科学]