黑龙江省水稻穗颈瘟人工接种技术研究  

Research on inoculation system for rice neck blast evaluation in Heilongjiang province

作　　者：马军韬[1] 张国民[1] 张丽艳[1] 邓凌韦[1] 王永力[1] 高洪儒[1] 宫秀杰[2] 赵文生 Ma Juntao;Zhang Guomin;Zhang Liyan;Deng Lingwei;Wang Yongli;Gao Hongru;Gong Xiujie;Zhao Wensheng(Institute of Biotechnology,Heilongjiang Academy of Agricultural Sciences/Northern Japonica Rice Molecular Breeding Joint Research Center,Chinese Academy of Science,Harbin Heilongjiang 150028,China;Instiute of Crop Cultivation and Tillage,Heilongiang Academy of Agricultural Sciences,Harbin Heilongjiang 150028,China;College of Plant Protecion,China Agricultural Univrsity,Beijing 100193,China)

机构地区：[1]黑龙江省农业科学院生物技术研究所/中国科学院北方粳稻分子育种联合研究中心,黑龙江哈尔滨150028 [2]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150028 [3]中国农业大学植物保护学院,北京100193

出　　处：《中国植保导刊》2021年第1期10-14,52,共6页China Plant Protection

基　　金：国家重点研发计划(2018YFD0200202-5);黑龙江省自然科学基金面上项目(C2015032)。

摘　　要：为建立水稻穗颈瘟准确的鉴定技术,在黑龙江省人工气候室完成发病条件鉴定试验,结合田间病圃完成接种条件鉴定试验。结果显示,在人工气候室内,自然光源下、环境温度26℃、相对湿度≥90%、水稻孕穗末期用5号或6号针头注射接种的鉴定效果最佳,供试水稻品种平均发病率为92.22%。在人工控制环境条件的前提下,试验点田间接种的效果也较好,供试水稻品种平均发病率在91.00%之上,证明该接种技术在田间同样适用。To develop an efficient inoculation system to identify rice neck blast in Heilongjiang Province, we studied the inoculation methods and environmental conditions both in the climate chamber and in the field. The main results were as follows: under the conditions of natural light, 26 ℃, relative humidity≥90% in the climate chamber, boot injection with five or six-gauge syringe needles in the late stage of booting achieved the optimal inoculation effect. The average incidence of tested rice cultivar was 92.22%. Moreover, the average incidence of tested rice cultivar in the field was above 91.00%and the inoculation effect was also good under controlled environmental conditions. It is proved that the inoculation technique of boot injection was also applicable for evaluating rice neck blast in the field.

关 键 词：水稻 穗颈瘟 人工接种 发病条件 接种条件 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]