骆驼蓬碱通过LOXL1-AS1影响卵巢癌细胞CAOV3增殖、凋亡、迁移侵袭  

Harmine Affects the Proliferation,Apoptosis,Migration and Invasion of Ovarian Cancer Cell CAOV3 through LOXL1-AS1

作　　者：李贺月 张尊胜 王军 LI Heyue;ZHANG Zunsheng;WANG Jun(Department of Gynaecology,the Seventh People’s Hospital of Shanghai,Shanghai 200137,China)

机构地区：[1]上海市第七人民医院妇科,上海200137

出　　处：《中国细胞生物学学报》2021年第2期385-393,共9页Chinese Journal of Cell Biology

摘　　要：该研究探讨了骆驼蓬碱对卵巢癌细胞CAOV3增殖、凋亡、迁移侵袭的影响及分子机制。卵巢癌细胞CAOV3分为对照组,骆驼蓬碱低、中、高剂量组,si-NC组,si-LOXL1-AS1组,骆驼蓬碱高剂量+pcDNA组,骆驼蓬碱高剂量+pcDNA-LOXL1-AS1组。用细胞计数试剂盒-8(CCK-8)检测细胞存活率;平板克隆实验检测细胞克隆形成数;流式细胞术检测细胞凋亡;蛋白质印迹(Western blot)法检测蛋白表达;Transwell检测细胞迁移和侵袭;实时荧光定量PCR(RT-qPCR)检测LOXL1-AS1表达水平。结果显示,不同剂量骆驼蓬碱处理的CAOV3细胞中细胞存活率逐渐降低,克隆形成数逐渐减少,细胞凋亡率逐渐升高,Cleaved-caspase3表达水平逐渐升高,pro-caspase3表达水平逐渐降低,细胞迁移侵袭数逐渐减少,LOXL1-AS1表达水平逐渐降低(P<0.05)。干扰LOXL1-AS1表达后,细胞存活率降低,克隆形成数减少,细胞迁移侵袭数减少,细胞凋亡率升高,Cleaved-caspase3表达水平升高,pro-caspase3表达水平降低(P<0.05)。LOXL1-AS1过表达可减弱骆驼蓬碱对CAOV3细胞增殖、凋亡、迁移侵袭的影响。该研究得出,骆驼蓬碱通过下调LOXL1-AS1表达抑制卵巢癌细胞CAOV3增殖、迁移侵袭,促进凋亡。This work was to explore the effect and molecular mechanism of harmine on the proliferation,apoptosis,migration and invasion of ovarian cancer cell CAOV3.Ovarian cancer cells CAOV3 were divided into control group,harmine low,medium,and high dose groups,si-NC group,si-LOXL1-AS1 group,harmine high dose+pcDNA group,harmine high dose+pcDNA-LOXL1-AS1 group.CCK-8(cell counting kit-8)was uesd to detect cell viability;plate cloning test was uesd to detect the number of cell clones;flow cytometry was uesd to detect cell apoptosis;Western blot was uesd to detect protein expression;Transwell was uesd to detect cell migration and invasion;RT-qPCR(real-time fluorescent quantitative PCR)was uesd to detect the expression of LOXL1-AS1.The results showed that the cell survival rate of CAOV3 cells treated with different doses of harmine,the number of clone formation,cell migration and invasion,the expression of pro-caspase3 and LOXL1-AS1 were gradually decreased(P<0.05);the apoptosis rate and the expression of Cleaved-caspase3 were gradually increased.After interfering with the expression of LOXL1-AS1,cell survival rate,the number of clone formation,cell migration and invasion,the expression of pro-caspase3 were decreased(P<0.05);the cell apoptosis rate and the expression of Cleaved-caspase3 were increased.Overexpression of LOXL1-AS1 could attenuate the effects of harmine on the proliferation,apoptosis,migration and invasion of CAOV3 cells.This study showed that harmine could inhibit the proliferation,migration and invasion of ovarian cancer cell CAOV3 and promote apoptosis by down-regulating the expression of LOXL1-AS1.

关 键 词：骆驼蓬碱 LOXL1-AS1 卵巢癌 增殖 凋亡 迁移 侵袭 

分 类 号：R285.5[医药卫生—中药学]