艾灸对腹泻型肠易激综合征模型大鼠结肠及下丘脑组织中P物质、血管活性肠肽表达的影响  

Effects of moxibustion on substance P and vasoactive intestinal peptide expression in colon and hypothalamus tissues of model rats with diarrhea-predominant irritable bowel syndrome

作　　者：陈霞 王宇 仝理 吴立斌 李难[2] 储浩然[2] CHEN Xia;WANG Yu;TONG Li;WU Libin;LI Nan;CHU Haoran(School of Postgraduate,Anhui University of Chinese Medicine,Hefei,Anhui,230038,China;Famous Doctor Hall,the Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei,Anhui,230061,China)

机构地区：[1]安徽中医药大学研究生院,安徽合肥230038 [2]安徽中医药大学第二附属医院名医堂,安徽合肥230061

出　　处：《甘肃中医药大学学报》2021年第1期1-5,共5页Journal of Gansu University of Chinese Medicine

基　　金：国家自然科学基金面上基金项目(81774399);安徽省名老中医储浩然工作室建设项目(卫办秘〔2015〕404号)。

摘　　要：目的观察艾灸对腹泻型肠易激综合征(IBS-D)模型大鼠结肠及下丘脑组织中P物质(SP)、血管活性肠肽(VIP)表达的影响。方法将36只SPF级健康SD雄性大鼠采用随机数字表法分为空白组、模型组、艾灸组3组,每组12只。除空白组外,艾灸组和模型组大鼠采用慢性束缚联合番泻叶溶液灌胃的方法制备IBS-D模型。艾灸组采用艾条温和灸干预治疗,穴位选取两侧天枢和上巨虚,每日灸30 min,连续治疗14 d;空白组和模型组不采取任何干预措施。干预结束后,采集各组大鼠的结肠及下丘脑组织,采用反转录聚合酶链反应(RT-PCR)法检测结肠及下丘脑组织中的SP、VIP的相对表达量;于造模结束后和干预结束后分别计算各组大鼠当日的稀便率,并进行比较。结果与空白组比较,模型组大鼠结肠及下丘脑组织中的SP、VIP相对表达量均明显升高,差异有统计学意义(P<0.01);与模型组比较,艾灸组大鼠结肠及下丘脑组织中的SP、VIP相对表达量均明显降低,差异有统计学意义(P<0.01)。干预后,与模型组比较,艾灸组大鼠稀便率明显下降,差异有统计学意义(P<0.01)。结论艾灸天枢、上巨虚穴可降低IBS-D模型大鼠结肠及下丘脑组织中的SP、VIP表达水平,从而延缓痛觉传递、减轻炎症反应、调节胃肠功能紊乱,这可能是艾灸治疗IBS-D的作用机制之一。Objective To observe the effects of moxibustion on substance P(SP)and vasoactive intestinal peptide(VIP)expression in colon and hypothalamus tissues of model rats with diarrhea-predominant irritable bowel syndrome(IBS-D).Methods Thirty six SPF healthy male SD rats were divided into blank group,model group and moxibustion group according to random number table method,with 12 rats in each group.Except the blank group,rats in moxibustion group and model group were given chronic bound combined with intragastric administration of Sennae folium solution to prepare IBS-D model.The moxibustion group was given intervention treatment of warm moxibustion with a moxa-stick,bilateral acupoints Tianshu(St 25)and Shangjuxu(St 37)were selected,moxibustion for 30 minutes per day,continuous treatment for 14 days;while none of the intervention measures were taken to the blank group and model group.After the intervention,the colon and hypothalamus tissues of rats in each group were collected,and the relative expression quantity of SP and VIP therein were detected by reverse transcription polymerase chain reaction(RT-PCR)method.The loose stool rate of rats in each group on that very day was calculated and compared at the end of modeling and intervention.Results Compared with the blank group,the relative expression quantity of SP and VIP in the colon and hypothalamus of the model group significantly increased,and the difference was statistically significant(P<0.01);compared with the model group,the relative expression quantity of SP and VIP in the colon and hypothalamus of the moxibustion group significantly decreased,and the difference was statistically significant(P<0.01).After the intervention,compared with the model group,the loose stool rate of the moxibustion group decreased significantly,and the difference was statistically significant(P<0.01).Conclusion Moxibustion at acupoints of Tianshu(St 25)and Shangjuxu(St 37)can reduce the expression levels of SP and VIP in colon and hypothalamus of IBS-D model rats,thus delaying pain tr

关 键 词：腹泻型肠易激综合征 艾灸 天枢穴 上巨虚穴 P物质 血管活性肠肽 稀便率 大鼠 实验研究 

分 类 号：R245.81[医药卫生—针灸推拿学] R574.4[医药卫生—中医临床基础]