健胃清肠合剂对不完全性肠梗阻模型大鼠肠黏膜机械屏障功能的影响  

Effects of Jianwei Qingchang Heji(健胃清肠合剂)on intestinal mucosal mechanical barrier function in model rats with incomplete intestinal obstruction

作　　者：徐中 张旭彤 任彦彦 吕巧莹 刘立德 李彦龙[1] 李生财[3] XU Zhong;ZHANG Xutong;REN Yanyan;LYU Qiaoying;LIU Lide;LI Yanlong;LI Shengcai(Department of Spleen and Stomach Diseases,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou,Gansu,730050,China;Clinical College of TCM,Gansu University of Chinese Medicine,Lanzhou,Gansu,730101,China;Department of Spleen and Stomach Diseases,Gansu Provincial Institute of Traditional Chinese Medicine,Lanzhou,Gansu,730050,China)

机构地区：[1]甘肃省中医院脾胃病科,甘肃兰州730050 [2]甘肃中医药大学中医临床学院,甘肃兰州730101 [3]甘肃省中医药研究院脾胃病科,甘肃兰州730050

出　　处：《甘肃中医药大学学报》2021年第1期11-15,共5页Journal of Gansu University of Chinese Medicine

基　　金：甘肃省中医药管理局项目(GZK-2017-56)。

摘　　要：目的研究健胃清肠合剂对不完全性肠梗阻模型大鼠血浆内毒素(ET)以及血清D-乳酸、二胺氧化酶(DAO)、肿瘤坏死因子-α(TNF-α)的影响。方法将112只SPF级Wistar大鼠采用随机数字表法分为空白组,假手术组,模型组,阳性对照组,健胃清肠合剂高、中、低剂量组7组,每组16只。采用回肠末端结扎法建立大鼠不完全性肠梗阻模型,各组给予相应药物或蒸馏水灌胃3 d。于末次灌胃1 h后股动脉取血,分别采用鲎试验偶氮基质显色法、酶偶联显色法、比色法、放射免疫法检测各组大鼠血浆ET含量以及血清D-乳酸含量、DAO活性、TNF-α表达量,并进行比较。结果与空白组比较,假手术组大鼠血浆ET含量以及血清D-乳酸含量、DAO活性、TNF-α表达量无明显变化,差异均无统计学意义(P>0.05);与空白组、假手术组比较,模型组大鼠各项指标均明显升高,差异有统计学意义(P<0.05);与模型组比较,阳性对照组,健胃清肠合剂高、中、低剂量组大鼠各项指标均明显降低,差异有统计学意义(P<0.05);与阳性对照组比较,健胃清肠合剂高、中剂量组大鼠各项指标均明显降低,差异有统计学意义(P<0.05);健胃清肠合剂各剂量组之间比较,高、中剂量组各项指标均明显低于低剂量组,差异有统计学意义(P<0.05)。结论健胃清肠合剂能够降低不完全性肠梗阻模型大鼠血浆ET含量及血清D-乳酸含量、DAO活性,有效抑制TNF-α的释放,具有肠黏膜机械屏障保护功能。Objective To study the effects of Jianwei Qingchang Heji(健胃清肠合剂)on plasma endotoxin(ET),D-lactic acid,diamine oxidase(DAO),and tumor necrosis factor-α(TNF-α)in incomplete intestinal obstruction models rats.Methods Totally 112 SPF Wistar rats were divided into 7 groups according to random number table method,including blank group,sham operation group,model group,positive control group,Jianwei Qingchang Heji high,medium and low dose group,with 16 rats in each.Incomplete intestinal obstruction model was established by ligation of the terminal ileum in rats,each group was given corresponding medicinal or intragastric administration of distilled water for 3 days.One hour after the last intragastric administration,the blood was taken from femoral artery.The content of plasma ET,the content of D-lactic acid,the activity of DAO and the expression of TNF-αin serum were determined by limulus ameobatic chromogenic assay,enzyme coupled chromogenic assay,colorimetry and radioimmunoassay,and then comparisons were made.Results Compared with the blank group,there were no significant changes in plasma ET content,serum D-lactic acid content,DAO activity and TNF-αexpression in the sham operation group,and the difference was not statistically significant(P>0.05);compared with the blank group and the sham operation group,the indexes of the model group significantly increased,and the difference was statistically significant(P<0.05);compared with the model group,the indexes of the positive control group,Jianwei Qingchang Heji high,medium and low dose group significantly decreased,and the difference was statistically significant(P<0.05);compared with the positive control group,the indexes of rats in Jianwei Qingchang Heji high and medium dose group significantly decreased,and the difference was statistically significant(P<0.05);compared among Jianwei Qingchang Heji various dose groups,the indexes of Jianwei Qingchang Heji high and medium dose group were significantly lower than those of the low dose group,and the differe

关 键 词：不完全性肠梗阻 健胃清肠合剂 内毒素 D-乳酸 二胺氧化酶 肿瘤坏死因子-α 肠黏膜机械屏障 大鼠 实验研究 

分 类 号：R285.5[医药卫生—中药学]