中心复合设计-响应面法优化当归新品种“岷归3号”当归多糖提取工艺  

Optimization of extraction process of polysaccharides from new varieties of Angelica sinensis“mingui No.3”by central composite design response surface methodology

作　　者：周菲[1] 李硕 成小璐 俱蓉 李响 ZHOU Fei;LI Shuo;CHENG Xiaolu;JU Rong;LI Xiang(School of Information Engineering,Gansu University of Chinese Medicine,Lanzhou,Gansu,730101,China;School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou,Gansu,730101,China;Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine and Tibetan Medicine of the Universities of Gansu Province,Lanzhou,Gansu,730000,China;Cultivation Base of Key Laboratory for Chinese Materia Medica Quality and Standard Research of Gansu Province,Lanzhou,Gansu,730000,China)

机构地区：[1]甘肃中医药大学信息工程学院,甘肃兰州730101 [2]甘肃中医药大学药学院,甘肃兰州730101 [3]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [4]甘肃省中药质量与标准研究重点实验室培育基地,甘肃兰州730000

出　　处：《甘肃中医药大学学报》2021年第1期37-42,共6页Journal of Gansu University of Chinese Medicine

基　　金：国家自然科学基金地区基金项目(81960713);甘肃省中医药管理局项目(GZK-2017-7);甘肃省自然科学基金项目(18JR3RA201);甘肃省高校中(藏)药化学与质量研究省级重点实验室项目(zzy-2018-06);甘肃中医药大学青年基金项目(ZQ2015-21)。

摘　　要：目的采用中心复合设计-响应面法优化当归新品种“岷归3号”当归多糖的提取工艺。方法以“岷归3号”中当归多糖含量为评价指标,在单因素试验的基础上,采用中心复合设计-响应面分析法优化“岷归3号”当归多糖的提取工艺。结果“岷归3号”当归多糖的最佳提取工艺为:乙醇体积分数66%,提取时间120 min,液料比7∶1,当归多糖得率为8.67%。结论该方法合理、可行,可作为“岷归3号”当归多糖的提取工艺,也可为“岷归3号”的品质评价及推广应用提供参考。Objective To optimize the extraction process of polysaccharides from new varieties of Angelica sinensis“mingui No.3”by central composite design-response surface method.Methods Taking the polysaccharides content of new varieties of Angelica sinensis“mingui No.3”as the evaluation index,on the basis of single factor,the extraction process of“mingui No.3”polysaccharides was optimized by the method of central composite design-response surface methodology.Results The optimal extraction process of“mingui No.3”polysaccharides was∶the alcohol volume fraction was 66%,extraction duration was 120 min,and the ratio of material to liquid was 7∶1,the extraction rate of polysaccharides from Angelica sinensis was 8.67%.Conclusion This method was reasonable and feasible,and it can be used as the extraction process of polysaccharides from Angelica sinensis“mingui No.3”,can also provide a reference for the quality evaluation and application of Angelica sinensis“mingui No.3”.

关 键 词：“岷归3号” 中心复合设计-响应面分析法 当归多糖 提取工艺 

分 类 号：R284.2[医药卫生—中药学]