温度对序批式膜生物反应器生物脱氮影响及微生物机制解析  

Effects of temperature on biological nitrogen removal in batch membrane bioreactor and the microbial community mechanism

作　　者：陈彦霖 隋倩雯[1,2] 王拓 海永龙 郁达伟[1,2] 陈梅雪[1,2] 魏源送[1,2,3,4] CHEN Yanlin;SUI Qianwen;WANG Tuo;HAI Yonglong;YU Dawei;CHEN Meixue;WEI Yuansong(State Key Joint Laboratory of Environment Simulation and Pollution Control,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Department of Water Pollution Control Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;University of Chinese Academy of Sciences,Beijing 100049,China;Institute of Energy,Jiangxi Academy of Sciences,Nanchang 330029,China)

机构地区：[1]中国科学院生态环境研究中心,环境模拟与污染控制国家重点联合实验室,北京100085 [2]中国科学院生态环境研究中心,水污染控制实验室,北京100085 [3]中国科学院大学,北京100049 [4]江西省科学院能源研究所,南昌330029

出　　处：《环境工程学报》2021年第1期65-73,共9页Chinese Journal of Environmental Engineering

基　　金：国家重点研发计划课题(2016YFD0501405);江西省重点研发计划项目(20171ACG70018)。

摘　　要：针对生猪养殖废水处理过程中普遍存在的脱氮效果不佳,工艺流程较长等问题,采用中试规模序批式膜生物反应器(SMBR)处理生猪养殖废水,并与猪场现有废水处理A^(2)/O工艺进行了比较。结果表明:SMBR在运行温度逐步降低的情况下,可以取得较好的污染物去除效果,氨氮平均出水浓度为10 mg·L^(-1),去除率达到98.6%;总氮平均出水浓度为31 mg·L^(-1),去除率达到96.5%;COD为332 mg·L^(-1),去除率达到96.5%。猪场现有A^(2)/O工艺对氨氮、总氮和耗氧有机物的去除率分别为99%、88%和97%。高通量测序和OUT分类结果表明,SMBR中主要的AOB为Nitrosomonas,NOB主要为Nitrospira、Nitrobacter和Nitrolancea。AOB是SMBR主要的硝化细菌,NOB是A^(2)/O工艺中主要的硝化细菌,并且反硝化细菌在SMBR中的菌群丰度高于A^(2)/O工艺中的反硝化菌群丰度。综上所述,与A^(2)/O工艺相比,SMBR具有短流程和脱氮效果较好等优势,这可使其具有较好的应用前景。Aiming at solving poor nitrogen removal performance and long process flow of swine wastewater treatment,a pilot scale sequencing batch membrane biological reactor(SMBR)was used to treat the swine wastewater in comparison with the current used A^(2)/O treatment process.The results showed that good pollutant removals occurred even when the operating temperature of the SMBR decreased.The average effluent concentrations of ammonia,total nitrogen and COD were 10,31 and 332 mg·L^(-1),and their removal rates were98.6%,96.5%and 96.5%,respectively;while their corresponding removal rates by A^(2)/O were 99%,88%and97%,respectively.High-throughput sequencing and OTU classification showed that the major Ammoniaoxidizing bacteria(AOB)in the SMBR were Nitrosomonas,and the nitrite-oxidizing bacteria(NOB)were mainly Nitrospira,Nitrobacter and Nitrolancea.And AOB was the main nitrifying bacteria in the SMBR,NOB was the main nitrifying bacteria in the A^(2)/O process,and the abundance of denitrifying bacteria in the SMBR was higher than that in the A^(2)/O process.Thus,compared with the A^(2)/O,SMBR had the advantages of the short process and better biological nitrogen removal efficiency,which makes it have more application prospect.

关 键 词：生猪养殖废水 序批式膜生物反应器 A2/O 生物脱氮 温度 

分 类 号：X523[环境科学与工程—环境工程]