2018年杭州市登革热流行病学及病原特征研究  

Epidemiological and etiological characteristics of dengue fever in Hangzhou in 2018

作　　者：文艳苹[1] 汪皓秋[1] 于新芬[1] 张国忠[1] 周银燕[1] 钱昕[1] Wen Yanping;Wang Haoqiu;Yu Xinfen;Zhang Guozhong;Zhou Yinyan;Qian Xin(Sanitary Inspection Center,Hangzhou Centers for Disease Control and Prevention,Hangzhou 310012,China)

机构地区：[1]杭州市疾病预防控制中心卫生检验中心,310012

出　　处：《中华微生物学和免疫学杂志》2021年第2期119-125,共7页Chinese Journal of Microbiology and Immunology

基　　金：浙江省医药卫生科技计划项目面上项目(2019KY146,2020KY235)。

摘　　要：目的了解2018年杭州市登革热疫情的流行病学及病原特征。方法应用RT-PCR方法检测血清标本中的登革病毒核酸及其型别,并对阳性标本进行病毒分离、E基因扩增、序列测定及进化分析;描述病例的时间、人群和地区分布特征。结果2018年杭州市共检测登革热病例80例,其中输入性病例55例,本地病例25例(DENV-124例,DENV-31例)。本地病例集中在7月下旬—10月上旬,68%的病例年龄在50岁以上。E基因进化分析表明,本地病例余杭分离株、江干-上城分离株、钱塘新区分离株均属DENV-1基因Ⅰ型,分别与2015年印度尼西亚毒株、2017年缅甸毒株、2018年宁波毒株及2018年杭州的泰国输入性病例毒株最接近。另一DENV-3本地毒株为基因Ⅲ型,与2013年新加坡毒株同源性最高,核苷酸序列一致性为99.5%。结论杭州市登革热流行以输入性病例为主。输入病例引起本地暴发的风险较高,疫情呈多点输入引起多点小规模暴发的特点。Objective To analyze the epidemiological and etiological characteristics of dengue fever in Hangzhou in 2018.Methods RT-PCR was used to detect the nucleic acids and analyze the serotypes of dengue viruses(DENV)in serum samples collected from dengue fever cases.Phylogenetic trees based on the E gene sequences of DENV isolated from the serum samples were then constructed and analyzed.Epidemiological characteristics of these dengue fever cases were analyzed.Results A total of 80 cases of dengue fever were detected in Hangzhou in 2018 with 55 imported cases and 25 indigenous cases(24 caused by DENV-1 and one by DENV-3).These indigenous cases mainly occurred during late July to early October with people above 50 years old accounting for 68%.Phylogenetic analysis showed that DENV-1 strains isolated from the indigenous cases in Yuhang,Jianggan-Shangcheng and Qiantang districts all belonged to genotypeⅠ,and were respectively closely related to the strains from Indonesia in 2015,Myanmar in 2017,Ningbo in 2018 and Hangzhou imported cases from Thailand in 2018.The indigenous DENV-3 strain belonged to genotypeⅢ,and shared 99.5%homology with the Singapore strain in 2013.Conclusions Imported cases accounted for a large fraction of the dengue fever cases in Hangzhou,which brought a high risk to indigenous outbreak.Due to multiple imported cases,the current epidemic presented a characteristic of multiple small-scale outbreaks.

关 键 词：登革热 登革病毒 E基因 进化分析 

分 类 号：R512.8[医药卫生—内科学] R181.3[医药卫生—临床医学]