Single-cell patterning and axis characterization in the murine and human definitive endoderm  

作　　者：Lin-Chen Li Xin Wang Zi-Ran Xu Yan-Chun Wang Ye Feng Liu Yang Wei-Lin Qiu Li Yang Xin-Xin Yu Jun Gu Cheng-Ran Xu 

机构地区：[1]Ministry of Education Key Laboratory of Cell Proliferation and Differentiation,College of Life Sciences,Department of Human Anatomy,Histology,and Embryology,School of Basic Medical Sciences,Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,China [2]Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China [3]Beijing Institute of Collaborative Innovation,Beijing 100094,China [4]PKU-Tsinghua-NIBS Graduate Program,Peking University,Beijing 100871,China [5]Haidian Maternal&Child Health Hospital,Beijing 100080,China

出　　处：《Cell Research》2021年第3期326-344,共19页细胞研究（英文版）

基　　金：This work was supported by the National Key R&D Program of China(2019YFA0801500);the National Basic Research Program of China(2015CB942800);the National Natural Science Foundation of China(31521004,31471358,and 31522036);funding from Peking-Tsinghua Center for Life Sciences.

摘　　要：Defining the precise regionalization of specified definitive endoderm progenitors is critical for understanding the mechanisms underlying the generation and regeneration of respiratory and digestive organs,yet the patterning of endoderm progenitors remains unresolved,particularly in humans.We performed single-cell RNA sequencing on endoderm cells during the early somitogenesis stages in mice and humans.We developed molecular criteria to define four major endoderm regions(foregut,lip of anterior intestinal portal,midgut,and hindgut)and their developmental pathways.We identified the cell subpopulations in each region and their spatial distributions and characterized key molecular features along the body axes.Dorsal and ventral pancreatic progenitors appear to originate from the midgut population and follow distinct pathways to develop into an identical cell type.Finally,we described the generally conserved endoderm patterning in humans and clear differences in dorsal cell distribution between species.Our study comprehensively defines single-cell endoderm patterning and provides novel insights into the spatiotemporal process that drives establishment of early endoderm domains.

关 键 词：ORGANS PRECISE specified 

分 类 号：Q2[生物学—细胞生物学]