TaSPX3基因VIGS沉默表达降低小麦对叶锈病(Puccinia recondite f.sp.tritici)的抗性  

Silencing the expression of TaSPX3 by VIGS decreased the resistance of wheat to leaf rust(Puccinia reconditef.sp.tritici)

作　　者：张蕊 李博 李旭 尚文静[1] 韩迎春[2] 程琨[1] 刘娜[1] 郑文明[1,2] ZHANG Rui;LI Bo;LI Xu;SHANG Wenjing;HAN Yingchun;CHENG Kun;LIU Na;ZHENG Wenming(College of Life Science,Henan Agricultural University,Zhengzhou 450002,China;Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of Wheat and Maize Crop,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]河南农业大学小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心,郑州450002

出　　处：《中国农业大学学报》2021年第1期26-32,共7页Journal of China Agricultural University

基　　金：河南省自然科学基金项目(182300410023,202300410202);河南省科技攻关项目(182102110278);河南省教育厅重点项目(17B210003)。

摘　　要：为挖掘TaSPX3基因在小麦抗叶锈病中的作用,本研究以前期对叶锈菌感染的小麦转录组数据分析发现的TaSPX3基因受到叶锈菌侵染诱导表达为依据,利用BSMV-VIGS系统沉默中国春和郑麦9023中的TaSPX3基因,利用qRT-PCR技术进一步分析小麦抗病相关基因的转录水平。结果发现TaSPX3基因沉默后,小麦叶锈菌感染加重,降低了小麦对叶锈病的抗性;同时,对侵染过程抗病相关基因转录检测发现,抗病相关基因PR2和CAT的表达水平显著下调。本研究表明TaSPX3基因可能通过调控PR2和CAT基因的表达参与小麦对叶锈病的抗性。In order to explore the function of TaSPX3 in wheat resistance to leaf rust based on the discover that TaSPX3 expression was induced by leaf rust infection,BSMV-VIGS system was used to silence TaSPX3 in Chinese Spring and Zhengmai 9023.qRT-PCR was adopted to analysis the expression of disease resistant genes.The results showed that:The disease severity of leaf rust on BSMV∶TaSPX3 infected wheat leaves was aggravated,which indicated that the resistance to leaf rust was reduced after silencing TaSPX3 gene;The expression levels of diseasesresistance genes PR2 and CAT were significantly down-regulated in the leaves.In conclusion,TaSPX3 might be involved in wheat resistance to leaf rust fungus by regulating the expression of PR2 and CAT genes.

关 键 词：小麦 TaSPX3 VIGS 叶锈病 抗病性 

分 类 号：S512[农业科学—作物学]