褪黑素对内蒙古绒山羊皮肤上皮细胞增殖及wnt10b和β-catenin基因表达的影响  

Effects of melatonin on the proliferation of skin epithelial cells and expressions of Wnt10b and β-catenin genes in Inner Mongolian cashmere goats

作　　者：吴子贤 吴静 宫文典 卢泽宇 赵飞飞 刘俊阳 杨德智 张燕军[1] 苏蕊[1] 赵艳红[1] 刘佳森 WU Zixian;WU Jing;GONG Wendian;LU Zeyu;ZHAO Feifei;LIU Junyang;YANG Dezhi;ZHANG Yanjun;SU Rui;ZHAO Yanhong;LIU Jiasen(College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031,China;Inner Mongolia Wulanchabu City Shangdu County Vocational and Technical School,Ulanqab 013450,China)

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古自治区农牧业科学院畜牧研究所,呼和浩特010031 [3]内蒙古乌兰察布市商都县职业技术学校,内蒙古乌兰察布013450

出　　处：《中国农业大学学报》2021年第1期62-68,共7页Journal of China Agricultural University

基　　金：国家自然科学基金项目(31860628、31460588、31560624);内蒙古自然基金项目(2020LH03020、2015MS0381、2017MS0304);内蒙古农牧业科技创新基金项目(2017CXJJM01-3)。

摘　　要：为研究褪黑素对内蒙古绒山羊皮肤上皮细胞生长的作用模式,将褪黑素浓度设置为0、100、300、600和900pg/mL 5个试验组,利用CCK-8法检测各组皮肤上皮细胞增殖情况,实时荧光定量PCR检测Wnt10b和β-catenin基因的表达,ELISA检测Wnt10b及β-catenin蛋白的表达。结果表明:300pg/mL褪黑素对皮肤上皮细胞增殖效果最佳(P<0.05),Wnt10b和β-catenin的mRNA和蛋白质表达量极显著高于其他组(P<0.01)。综上,300pg/mL的褪黑素可显著促进皮肤上皮细胞增殖,且显著提高Wnt10b和β-catenin的表达。In order to study the effect of melatonin on the growth of skin epithelial cells of Inner Mongolia cashmere goats,five test groups were set at 0,100,300,600 and 900 pg/mL of melatonin.CCK-8 method was used to detect the proliferation of skin epithelial cells.Quantitative PCR was to detect the expression of Wnt10 bandβ-catenin gene,and ELISA was used to detect the expression of Wnt10 bandβ-catenin protein.The results showed that 300 pg/mL melatonin had the best effect on the proliferation of skin epithelial cells(P<0.05),and the mRNA and protein expressions of Wnt10 bandβ-catenin were significantly higher than those in other groups(P<0.01).In summary,300 pg/mL melatonin can significantly promote the proliferation of skin epithelial cells and increase the expressions of Wnt10 bandβ-catenin.

关 键 词：内蒙古绒山羊 褪黑素 皮肤上皮细胞 细胞增殖 Wnt10b Β-CATENIN 

分 类 号：S813.3[农业科学—畜牧学]