不同浓度植物根系分泌物微生态效应研究  

Study on Microecological Effects of Root Exudates of Garden Plantswith Different Concentrations

作　　者：沈凤英[1,2] 吴伟刚[2] 李亚宁[1] 刘大群[1,3] 滕云 SHEN Fengying;WU Weigang;LI Yaning;LIU Daqun;TENG Yun(College of Plant Protection,Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071001,China;College of Agriculture and Forestry Science and Technology,North University of Hebei,Zhangjiakou 075000,China;Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]国家北方山区农业工程技术研究中心/河北省植物病虫害生物防治工程技术研究中心/河北农业大学植物保护学院,河北保定071001 [2]河北北方学院农林科技学院,河北张家口075000 [3]中国农业科学院研究生院,北京100081

出　　处：《生态环境学报》2021年第2期313-319,共7页Ecology and Environmental Sciences

基　　金：河北北方学院校级青年基金项目(QN2018004);河北省教育厅科技指导项目(Z2019071);河北省教育厅科技青年基金项目(QN2019120);张家口市2019年市级科技计划财政资助项目科技特派员专项项目(1911016C-7);张家口市2019年市级科技计划财政资助项目科技特派员专项项目(1911016C-10)。

摘　　要：探讨夹竹桃(Nerium oleander)根系分泌物对土壤微生物量碳、呼吸强度等微生物学特征及土壤微生物群落功能多样性的影响,深入揭示夹竹桃根系分泌物的微生态效应,通过向土壤中添加植物根系分泌物溶液的方法,研究了不同浓度(CK,15 mL去离子水作为对照;LC,5 mL分泌物+10 mL去离子水;MC,10 mL分泌物+5 mL去离子水;HC,15 mL分泌物)的外源植物根系分泌物对土壤理化性质、土壤微生物群落功能多样性、微生物量碳、微生物熵、基础呼吸和代谢熵的影响。结果表明,不同浓度根系分泌物处理pH值显著低于CK(P<0.05);有机碳、含水量、全氮、碱解氮、速效磷含量显著高于CK(P<0.05),其大小表现为HC>MC>LC>CK;而土壤全磷无明显差异(P>0.05)。细菌、真菌、放线菌和微生物总数均显著高于对照(P<0.05),不同处理之间差异均显著(P<0.05),其大小表现为HC>MC>LC>CK。微生物量碳、微生物熵和基础呼吸均显著高于对照(P<0.05),不同处理之间差异均显著(P<0.05),其大小表现为HC>MC>LC>CK。土壤代谢熵表现为HC>MC>LC>CK,HC和MC差异不显著(P>0.05)。平均吸光值、物种丰富度指数、Mcintosh指数均显著高于对照(P<0.05),不同处理之间差异均显著(P<0.05),其大小表现为HC>MC>LC>CK。不同处理间优势度指数差异不显著(P>0.05)。主成分分析表明:中浓度根系分泌物处理的土壤与CK土壤微生物群落代谢特征相近,其明显不同于高浓度根系分泌物处理的土壤,说明它们对单一碳源的利用能力不同,其群落代谢特征存在差异。总体来看,高浓度的根系分泌物能够显著改变土壤微生物学特性及群落功能多样性。To investigate the effects of root exudates of Nerium Oleander on soil microbial biomass carbon,respiration intensity and functional diversity of soil microbial community,and to further reveal the microecological effects of root exudates of Nerium Oleander.Studied the different concentration(CK[15 mL deionized water as control],LC+10 mL deionized water][5 mL secretion,MC+5 mL deionized water][10 mL secretion,HC][15 mL secretion)of exogenous plant root secretion on soil physical and chemical properties,soil microbial community functional diversity,microbial biomass carbon,microbial entropy,the influence of the respiratory and metabolic entropy.The results showed that the pH value of root exudates treated with different concentrations was significantly lower than CK(P<0.05).The contents of organic carbon,water content,total nitrogen,alkaloid-hydrolyzed nitrogen and available phosphorus in root exudates of different concentrations were significantly higher than CK(P<0.05).However,there was no significant difference in soil total phosphorus(P>0.05).The total number of bacteria,fungi,actinomycetes and microorganisms under the treatment of plant root exudates with different concentrations was significantly higher than that of the control group(P<0.05),and the difference among different treatments was significant(P<0.05).The size was shown as:HC>MC>LC>CK.Microbial biomass carbon,microbial entropy and basal respiration were significantly higher than those in the control group under the treatment of plant root exudates with different concentrations(P<0.05),and there were significant differences among different treatments(P<0.05).Their sizes were successively shown as:HC>MC>LC>CK.The metabolic entropy of soil showed HC>MC>LC>CK,and no significant difference between HC and MC(P>0.05).The average light absorption value,species richness index and Mcintosh index of plant root exudations of different concentrations were significantly higher than those of the control group(P<0.05),and the differences among different treatments w

关 键 词：夹竹桃 根系分泌物 微生物群落 功能多样性 

分 类 号：S154.36[农业科学—土壤学] X17[农业科学—农业基础科学]