内皮蛋白C受体对类风湿关节炎疾病进展的影响  

The effect of endothelin C receptor on the disease progression of rheumatoid arthritis

作　　者：刘汇洋 刘轩绮 白静茹 赵京洋 尹芳蕊[1,2] 王永福[1,2] LIU Huiyang;LIU Xuanqi;BAI Jingru;ZHAO Jingyang;YIN Fangrui;WANG Yongfu(The First Affiliated Hospital of Baotou Medical College,Baotou 014010,China;Baotou Medical College)

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010 [2]包头医学院

出　　处：《包头医学院学报》2021年第2期39-45,共7页Journal of Baotou Medical College

基　　金：内蒙古自治区自然科学项目基金(2020LH08001)。

摘　　要：目的:研究分析可溶性内皮细胞蛋白C受体(Endothelial cell protein C receptor,sEPCR)与膜型EPCR(mEPCR)在参与类风湿关节炎(Rheumatoid Arthritis,RA)疾病进展中的作用。方法:在DBA1/J小鼠尾部注射II型胶原蛋白诱导关节炎小鼠(CIA小鼠),并在两次胶原免疫期间给予实验组小鼠腹腔内注射sEPCR重组蛋白或EPCR腺病毒小干扰载体进行发病前干预;二次加强免疫后的第7 d,对CIA小鼠的关节红肿程度进行连续评分;第28 d处死小鼠,进一步行关节的组织病理学、放射学评估,评价sEPCR和mEPCR对RA疾病进展的作用。结果:(1)与对照组相比,给予sEPCR干预的CIA小鼠关节评分显著降低,组织学和放射学结果显示骨侵蚀程度也较轻;(2)与sEPCR重组蛋白干预相反,mEPCR组CIA小鼠由于EPCR表达下调,CIA小鼠表现为更严重的关节破坏和炎症。结论:EPCR的表达异常与RA的疾病进展密切相关;给予sEPCR治疗可延缓骨破坏,缓解RA的疾病进展;而mEPCR的表达水平降低会介导RA疾病的发生和发展。Objective:Endothelial cell protein C receptor(EPCR)is believed to be a transmembrane protein stored on the endothelial cell membrane and can be expressed in rheumatoid arthritis(RA)in the synovial tissue of patients.The research further analyzes the role of soluble EPCR and membrane EPCR in the progression of RA disease.Methods:Arthritis mice(CIA mice)were induced by injecting type II collagen into the tail of DBA1/J mice,and sEPCR recombinant protein or EPCR adenovirus small interference were injected into the mice of experimental group during the two collagen immunizations to carry intervention before the onset of onset;on the 7th day after the second booster immunization,the degree of joint redness and swelling of the CIA mice was continuously scored;the mice were sacrificed on the 28th day,and the joint histopathological and radiological evaluations were performed to evaluate sEPCR and mEPCR on the progression of RA disease.Results:(1)Compared with the control group,the joint scores of CIA mice treated with sEPCR were significantly lower,and the histological and radiological results showed that the degree of bone erosion was also lighter;(2)In contrast to the sEPCR recombinant protein intervention,CIA mice of the mEPCR group showed more severe joint destruction and inflammation due to the down-regulation of EPCR expression.Conclusion:The abnormal expression of EPCR is closely related to the disease progression of RA;sEPCR treatment can delay bone destruction and alleviate the disease progression of RA;and the decrease of mEPCR expression level can mediate the occurrence and development of RA.

关 键 词：类风湿关节炎 膜型EPCR 可溶性EPCR 

分 类 号：R28[医药卫生—中药学]