血红素氧化酶在脓毒症大鼠组织中的表达  

Expression of HO⁃1 mRNA and HO⁃1 in sepsis rats

作　　者：张凯[1] 李文军 ZHANG Kai;LI Wenjun(Department of Critical Care Med-icine,the Third Hospital of Affiliated Hospital of Northwest University,Xi′an 710016,China)

机构地区：[1]西北大学附属医院西安市第三医院重症医学科,西安710016

出　　处：《实用医学杂志》2021年第6期713-717,共5页The Journal of Practical Medicine

基　　金：陕西省社会发展科技攻关项目(编号:2016SF-106)。

摘　　要：目的研究脓毒症大鼠肺组织中血红素氧化酶(HO-1)和HO-1 mRNA的表达情况。方法选取40只大鼠,随机分为假手术组20只和脓毒症组20只;两组分别于术前20 min和造模后2、6 h和12 h进行断头处死大鼠,并制备HE染色光镜病理切片;收集Western印迹法HO1-1蛋白活性水平检测结果和Realtime PCR法对HO-1 mRNA水平的检测结果;通过大鼠盲肠结扎穿孔(CLP)方法构建脓毒症模型;免疫组化观察各时间点肺组织形态、HO-1 mRNA与HO-1蛋白水平的表达情况;观察脓毒症大鼠肺损伤组织形态、HO-1水平变化;分析HO-1与脓毒症中相应指标及肺功能损伤的关系。结果假手术组肺组织形态一直处于完整状态,而脓毒症组大鼠肺组织形态在肺组织发生了明显的病理变化;各时间点脓毒症组大鼠体内的HO-1印迹颜色均很深,在12 h印迹颜色最深,其次为6、24 h的颜色最浅;而假手术组在3个时间点的HO-1印迹颜色均很浅,且与脓毒症组一样在12 h印迹颜色最深,其次为6、24h的颜色最浅;假手术组一直保持平稳,未发生明显变化,而脓毒症组OD值在2 h最低,随后逐渐上升,经比较3个时间点内OD差异有统计学意义(P <0.05);脓毒症组大鼠OD值在各时间点中HO-1 mRNA水平均比假手术组高(P <0.05)。结论脓毒症组大鼠肺组织中HO-1的表达量明显高于假手术组,说明HO-1对脓毒症患者肺组织的病理形态学变化有明显影响;脓毒症组中肺组织的病理形态学损伤随着肺组织中HO-1的表达量的增加而逐渐减轻,说明HO-1对脓毒症患者的肺组织病理形态有明显的保护作用和改善效果。Objective To study the expression of heme oxidase(HO⁃1)and HO⁃1 mRNA in the lung tissue of septic rats.Methods Forty rats were selected and randomly divided into a sham operation group of 20 rats and a sepsis group of 20 rats;the two groups were decapitated and sacrificed at 20 min before surgery and 2,6 h and 12 h after modeling.HE stained light microscope pathological sections;collected the detection results of HO1⁃1 protein activity level by Western blotting method and the detection results of HO⁃1 mRNA level by Realtime PCR method;constructed sepsis model by rat cecal ligation and perforation(CLP)method;immune group Observe the lung tissue morphology,HO⁃1mRNA and HO⁃1 protein expression at each time point;observe the lung injury tissue morphology and HO⁃1 level changes in sepsis rats;analyze the corresponding indexes in HO⁃1 and sepsis And lung function damage.Results The lung tissue morphology of the sham operation group was always in a com⁃plete state,while the lung tissue morphology of the sepsis group had obvious pathological changes in the lung tissue;the color of HO⁃1 imprints in the sepsis group was very different at each time point.The color of the blot is darkest at 12 h,followed by the lightest color at 6,24 h;while the color of the HO⁃1 blot at the 3 time points of the sham operation group is very light,and the color of the blot at 12 h is the darkest at 12 h like the sepsis group.Followed by the lightest color at 6 and 24 h;the sham operation group has remained stable without significant changes,while the sepsis group has the lowest OD value at 2 h,and then gradually increases.The difference in OD at the 3 time points is statistically significant significance(P<0.05);the OD value of rats in the sepsis group was higher than that in the sham operation group at each time point(P<0.05).Conclusion The expression of HO⁃1 in the lung tissue of the sepsis group was significantly higher than that in the sham operation group,indicat⁃ing that HO⁃1 has a significant effect on the p

关 键 词：脓毒症 血红素氧合酶-1 大鼠 肺组织 HE染色 HO-1 mRNA 

分 类 号：R45[医药卫生—治疗学]