脑出血术后脑脊液中食管癌相关基因4和S100B蛋白表达水平与GCS评分及Barthel指数的相关性研究  

Expression Level of Esophageal Cancer-Related Gene 4 and S100B Protein in Cerebrospinal Fluid after Intracerebral Hemorrhage and Its Correlation with GCS Score and Barthel Index

作　　者：陈博[1] 朱娜[2] 徐翠香[3] 黄晓燕[3] 汪玲果[4] CHEN Bo;ZHU Na;XU Cui-xiang;HUANG Xiao-yan;WANG Ling-guo(Department of Neurosurgery,Shaanxi Provincial People’s Hospital,Xi’an 710068,China;Scientific Research Division Department,Shaanxi Provincial People’s Hospital,Xi’an 710068,China;Central Laboratory,Shaanxi Provincial Key Laboratory of Infection and Immune Diseases,Shaanxi Provincial People’s Hospital,Xi’an 710068,China;Department of Medical Affairs,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)

机构地区：[1]陕西省人民医院神经外科,西安710068 [2]陕西省人民医院科研处,西安710068 [3]陕西省人民医院中心实验室陕西省感染与免疫重点实验室,西安710068 [4]陕西省人民医院医务处,西安710068

出　　处：《现代检验医学杂志》2021年第2期11-14,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金(81271341),陕西省卫计委联合攻关项目[2016B004]。

摘　　要：目的探讨脑出血(intracerebral hemorrhage,ICH)患者术后脑脊液(CSF)中食管癌相关基因4(ECRG4)与S100钙结合蛋白B(S100B)表达水平与格拉斯哥昏迷评分(Glasgow coma scale,GCS)和巴氏指数(barthel index,BI)的相关性,及其在脑出血患者术后神经功能康复中的临床应用价值。方法选择2017年1月~2019年12月入住陕西省人民医院神经外科治疗的基底节区ICH患者30例,采用酶联免疫吸附法(ELISA)检测10例对照组、10例腰大池引流组和10例腰椎穿刺组患者ICH术后ECRG4与S100B蛋白水平,进行GCS和BI评分,并进行相关性分析。结果术后7天时,对照组ECRG4(1.14±0.39pg/ml)低于引流组(15.50±0.28 pg/ml)和穿刺组(10.71±0.89pg/ml),对照组S100B(0.550±0.310ng/ml)水平高于引流组(0.154±0.025ng/ml)与穿刺组(0.192±0.030ng/ml),引流组两者表达水平与穿刺组比较,差异均有统计学意义(t=11.630,28.120,均P<0.05)。术后7天时,引流组与穿刺组GCS(11.62±1.71,10.19±1.41)及BI(43.24±10.05,40.64±10.10)评分均高于对照组(GCS:7.07±1.43,BI:21.73±6.45),差异具有统计学意义(t=7.466,4.180,P<0.05)。结论ECRG4与S100B蛋白在脑出血患者脑脊液中的变化趋势与GCS和BI评分具有相关性,两者联合检测,有助于调整治疗方案,从而降低患者神经功能障碍的发生,改善预后。Objective To investigate the correlation between the expression levels of ECRG4 and S100B protein in CSF and Glasgow coma scale(GCS),barthel index(BI)in patients with intracerebral hemorrhage(ICH)after operation,and its clinical application value in the rehabilitation of neurological function after operation of cerebral hemorrhage.Methods The levels of ECRG4 and S100B protein were detected by enzyme-linked immunosorbent assay(ELISA)in 10 cases of control group,10 cases of lumbar cistern drainage group and 10 cases of lumbar puncture group.GCS and BI scores were performed,and the correlation was analyzed.Results On day 7 after operation,ECRG4 in control group(1.14±0.39 pg/ml)was lower than that in drainage group(15.50±0.28 pg/ml)and puncture group(10.71±0.89 pg/ml),S100B in control group(0.550±0.310 ng/ml)was higher than that in drainage group(0.154±0.025ng/ml)and in puncture group(0.192±0.030 ng/ml),and there was significant difference among the groups(t=11.630,28.120,P<0.05).The GCS and BI of drainage group(11.62±1.71,10.19±1.41)and puncture group(43.24±10.05,40.64±10.10)were significantly higher than those of control group(GCS:7.07±1.43,BI:21.73±6.45),the difference was statistically significant(t=7.466,4.180,P<0.05).Conclusion The change trend of ECRG4 and S100B protein in cerebrospinal fluid of patients with intracerebral hemorrhage were positively correlated with GCS and BI.The combined detection of two indicators is helpful to adjust the treatment plan,so as to reduce the occurrence of neurological dysfunction and improve the prognosis.

关 键 词：脑出血 食管癌相关基因4 S100钙结合蛋白B 脑脊液 

分 类 号：R651.11[医药卫生—外科学] R392.11[医药卫生—临床医学]