miR-21调控STAT3信号通路对肾母细胞瘤细胞增殖及迁移的影响  

Effects of miR-21 on STAT3 signaling pathway on proliferation and migration of nephroblastoma cells

作　　者：符策君[1] 吴丽珍[2] 陈泽婷 林海[1] 孙为增[1] Fu Cejun;Wu Lizhen;Chen Zeting(Dept of Pediatric Surgery,2Operating Room,Hainan Provincial People's Hospital,Haikou 570311)

机构地区：[1]海南省人民医院小儿外科,海口570311 [2]海南省人民医院手术室,海口570311

出　　处：《安徽医科大学学报》2021年第3期423-427,共5页Acta Universitatis Medicinalis Anhui

基　　金：海南自然科学基金面上项目(编号:20168283)。

摘　　要：目的探讨miR-21调控STAT3信号通路来对肾母细胞瘤细胞的增殖及迁移产生影响。方法RT-PCR测定人肾母细胞瘤组织以及癌旁组织中miR-21的表达。将人肾母细胞瘤细胞分为空白组、si-miR-21组(转染si-miR-21)和simiR-21 NC组(转染si-microRNA-21 NC),Western blot检测STAT3信号通路中相关蛋白和凋亡相关蛋白的表达,MTS实验检测细胞的增殖活性,流式细胞术检测细胞的凋亡,Transwell实验检测迁移能力。结果与癌旁组织相比,人肾母细胞瘤组织的miR-21相对表达量明显升高(P<0.05)。细胞转染后,3组p-STAT3、Bcl-2、Bcl-xl相对表达量、A490吸光度值以及细胞迁移数差异均有统计学意义(P<0.001),与空白组比较,si-miR-21组升高(P<0.05),si-miR-21 NC组下降(P<0.05)。三组细胞凋亡率差异有统计学意义(P<0.001),与空白组比较,si-miR-21组下降(P<0.05),simiR-21 NC组升高(P<0.05)。转染细胞miR-21与p-STAT3、Bcl-2及Bcl-xl呈正相关(r=0.824、0.778、0.837,P<0.001)。结论过表达miR-21促进肾母细胞瘤细胞的增殖及迁移作用,其机制可能是通过上调STAT3信号通路来实现。Objective To investigate the effect of miR-21 on STAT3 signaling pathway on the proliferation and migration of nephroblastoma cells.Methods The expression of miR-21 was detected by RT-PCR in human nephroblastoma and paracancerous tissues.Human nephroblastoma cells were divided into three groups:Blank group,simiR-21 group(transfected with si-miR-21)and si-miR-21 NC group(transfected with si-miR-21 NC).The expressions of related proteins and apoptosis related proteins in STAT3 signaling pathway were detected by Western blot.The cell proliferation activity was detected by MTS.The cell apoptosis was detected by flow cytometry,and the cell migration ability was detected by Transwell.Results The relative expression of miR-21 in human nephroblastoma was significantly higher than that in adjacent tissues(P<0.05).After transfection,there were significant differences in the relative expression of p-STAT3,Bcl-2,Bcl-xl,A490 absorbance value and cell migration number among the three groups(P<0.001).Compared with the Blank group,si-miR-21 group increased(P<0.05),simiR-21 NC group decreased(P<0.05).There were significant differences in cell apoptosis among the three groups(P<0.001).Compared with the Blank group,si-miR-21 group decreased(P<0.05),si-miR-21 NC group increased(P<0.05).miR-21 was positively correlated with p-STAT3,Bcl-2 and Bcl-xl(r=0.824,0.778,0.837;P<0.001).Conclusion Over expression of miR-21 can promote the proliferation and migration of nephroblastoma cells,which may be due to the up regulation of STAT3 signaling pathway.

关 键 词：MIR-21 STAT3信号通路 肾母细胞瘤 增殖 迁移 

分 类 号：Q812[生物学—生物工程]