Diet induced the change of mtDNA copy number and metabolism in Angus cattle  

作　　者：Ying Bai José A.Carrillo Yaokun Li Yanghua He Jiuzhou Song 

机构地区：[1]College of Life Sciences and Food Engineering,Hebei University of Engineering,Handan 056038,China [2].2Department of Animal&Avian Sciences,University of Maryland,College Park,MD 20742,USA [3]Council on Dairy Cattle Breeding,Bowie,MD 20716,USA [4].4Human Nutrition,Food and Animal Sciences,University of Hawaii at Manoa,Honolulu,HI 96822,USA.

出　　处：《Journal of Animal Science and Biotechnology》2021年第1期172-184,共13页畜牧与生物技术杂志（英文版）

基　　金：supported by Maryland Agricultural Experiment Station(MAES),Jorgensen Endowment Funds。

摘　　要：Background: Grass-fed and grain-fed Angus cattle differ in the diet regimes. However, the intricate mechanisms of different beef quality and other phenotypes induced by diet differences are still unclear. Diet affects mitochondrial function and dynamic behavior in response to changes in energy demand and supply. In this study, we examined the mtDNA copy number, mitochondria-related genes expression, and metabolic biomarkers in grass-fed and grainfed Angus cattle.Results: We found that the grass-fed group had a higher mtDNA copy number than the grain-fed group. Among different tissues, the mtDNA copy number was the highest in the liver than muscle, rumen, and spleen. Based on the transcriptome of the four tissues, a lower expression of mtDNA-encoded genes in the grass-fed group compared to the grain-fed group was discovered. For the mitochondria-related nuclear genes, however, most of them were significantly down-regulated in the muscle of the grass-fed group and up-regulated in the other three tissues. In which, COX6 A2, POLG2, PPIF, DCN, and NDUFA12, involving in ATP synthesis, mitochondrial replication,transcription, and maintenance, might contribute to the alterations of mtDNA copy number and gene expression.Meanwhile, 40 and 23 metabolic biomarkers were identified in the blood and muscle of the grain-fed group compared to a grass-fed group, respectively. Integrated analysis of the altered metabolites and gene expression revealed the high expression level of MDH1 in the grain-fed group might contribute to the mitochondrial NADH oxidation and spermidine metabolism for adapting the deletion mtDNA copy number.Conclusions: Overall, the study may provide further deep insight into the adaptive and regulatory modulations of the mitochondrial function in response to different feeding systems in Angus cattle.

关 键 词：Beef quality Different diet Gene expression METABOLISM MTDNA 

分 类 号：S823[农业科学—畜牧学]