The community structure and microbial linkage of rumen protozoa and methanogens in response to the addition of tea seed saponins in the diet of beef cattle  

作　　者：Cui Tan Carlos A.Ramírez-Restrepo Ali Mujtaba Shah Rui Hu Matt Bell Zhisheng Wang Chris McSweeney 

机构地区：[1]Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,Sichuan,China [2]Commonwealth Scientific and Industrial Research Organisation,CSIRO Agriculture and Food,Australian Tropical Sciences and Innovation Precinct,James Cook University,Townsville QLD 4811,Australia [3]Present address:CR Eco-efficient Agriculture Consultancy(CREAC),46 Bilbao Place,Bushland Beach QLD 4818,Australia [4]Department of Livestock Production,Shaheed Benazir Bhutto University of Veterinary and Animal Sciences,Sakrand,Sindh 67210,Pakistan [5]“Low Carbon Breeding Cattle and Safety Production”,University Key Laboratory of Sichuan Province,Ya’an 625014,Sichuan,China [6]School of Biosciences,University of Nottingham,Sutton Bonington,Loughborough LE125RD,UK [7]CSIRO Agriculture,Queensland BioScience Precinct,St Lucia,Brisbane QLD 4067,Australia.

出　　处：《Journal of Animal Science and Biotechnology》2021年第1期272-281,共10页畜牧与生物技术杂志（英文版）

基　　金：supported by grants from the Natural Science Foundation of China (31272472);project grants from Meat & Livestock Australia,Fisheries and Forestry in Australia and Commonwealth Scientific and Industrial Research Organization (CSIRO)。

摘　　要：Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 emission.Conclusions: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.

关 键 词：Beef steers Methane METHANOGEN PROTOZOA Tea seed saponin 

分 类 号：S823.5[农业科学—畜牧学]