利用KASP标记筛选含rhg1和Rhg4位点的大豆抗病资源  

Marker-Assisted Screening of Soybean Cyst Nematode Germplasms Harboring Resistance Loci rhg1 and Rhg4

作　　者：练云[1] 李海朝[1] 李金英[1] 周扬 王仕伟 张辉[1] 雷晨芳[1] 武永康[1] 张晶鹏 卢为国[1] LIAN Yun;LI Hai-chao;LI Jin-ying;ZHOU Yang;WANG Shi-wei;ZHANG Hui;LEI Chen-fang;WU Yong-kang;ZHANG Jin-peng;LU Wei-guo(Henan Academy of Crop Molecular Breeding Zhengzhou Subcenter of National Soybean Improvement Center/Key Laboratory of Oil Crops in Huanghuaihai Plains,Ministry of Agriculture and Rural Afairs/Henan Provincial Key Laboratory for Oil Crops Inprovement/Institute of Industrial Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002)

机构地区：[1]河南省作物分子育种研究院/国家大豆改良中心郑州分中心/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室/河南省农业科学院经济作物研究所,郑州450002

出　　处：《植物遗传资源学报》2021年第2期399-406,共8页Journal of Plant Genetic Resources

基　　金：国家重点研发项目(2017YFD0101400);河南省科技攻关项目(202102110158)。

摘　　要：大豆胞囊线虫(SCN,soybean cyst nematode)病是一种世界性大豆病害,培育抗SCN大豆品种是防治SCN的重要措施。本研究利用来自抗SCN主效位点rhg1和Rhg4的2个KASP标记,对487份大豆材料进行筛选,选择含有抗性位点且农艺性状优异的材料;通过室内接种大豆胞囊线虫2号、4号、5号生理小种和新小种X12,进行抗性鉴定验证其抗性水平,为培育抗病品种提供抗源材料。标记筛选结果表明,20份材料含有rhg1和Rhg4这2个主效抗性位点,其中,2份材料仅含有Rhg4位点。表型抗性鉴定结果表明,在接种的22份材料中,有1份材料对3个小种表现中抗,5份材料对2个小种表现抗或中抗。其中,1份材料对2号小种表现抗病、4份表现中抗;2份材料对4号小种表现中抗;4份材料对5号小种表现抗病、14份表现中抗;22份材料对新小种X12均表现出感病或中感。因此,本研究从487份材料中筛选出20份含有2个SCN抗性位点并具优异农艺性状的材料,可通过rhg1和Rhg4位点的累加培育抗病品种。Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most yield-limiting diseases of soybean worldwide.Releasing SCN-resistant cultivars are the primary basis for controlling SCN.Identification of parent lines showing major resistant loci and desirable agronomic characteristics are prerequisite for breeding resistant cultivars.In this study,a total of 487 soybean accessions were genotyped using two KASP markers that targeted to two resistance loci rhg1 and Rhg4.The accessions,which were identified harboring resistant alleles,were further subjected for resistance inoculated with race-2,race-4,race-5 and race-X12 in the greenhouse.Twenty accessions were characterized simultaneously harboring rhg1 and Rhg4 loci,while two accessions was found with the Rhg4 locus.Testing for SCN resistance suggested that one accession showed moderate resistant to three races and five accessions showed resistant or moderately resistant to two races.One accession was resistant and four accessions were moderately resistant to race-2.Two accessions showed moderately resistant to race-4.Four accessions showed resistant and fourteen moderately resistant to race-5.However,these accessions were turned out to be moderately susceptible or susceptible against race-X12.Collectively,by taking use of 487 soybean accessions this study identified 20 accessions that harbored two major resistant loci and showed desirable agronomic characteristics,might serve as elite parental lines in breeding for resistant cultivars via pyramiding rhg1 and Rhg4.

关 键 词：大豆 KASP标记 大豆胞囊线虫 rhg1 Rhg4 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]