SSR分子标记在橡胶树栽培品种鉴定中的应用  

Identification of Rubber Tree Clones Based on Simple Sequences Repeats Molecular Markers

作　　者：安泽伟[1] 曾霞[1] 胡彦师[1] 方家林[1] 程汉[1] 位明明[1] 黄华孙[1] AN Ze-wei;ZENG Xia;HU Yan-shi;FANG Jia-lin;CHENG Han;WEI Ming-ming;HUANG Hua-sun(State Centre for Rubber Breeding,Rubber Research Institute.Chinese Academy of Tropical Agriculural SciencesKey Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,Haikou 571101)

机构地区：[1]中国热带农业科学院橡胶研究所国家橡胶树育种中心/农业农村部橡胶树生物学与遗传资源利用重点实验室,海口571101

出　　处：《植物遗传资源学报》2021年第2期550-560,共11页Journal of Plant Genetic Resources

基　　金：中国热带农业科学院基本科研业务费专项资金(1630022020020,1630022020026);农业农村部作物种质资源保护项目(2130135)。

摘　　要：橡胶树是我国重要的热带经济作物,主要通过形态特征进行品种鉴定,但橡胶树品种间形态差异较小,品种鉴定难度较大。为建立一种快速、稳定且准确的橡胶树品种鉴定方法,本研究从426对橡胶树SSR引物中筛选出5对产物清晰且扩增稳定的引物组成核心引物对,利用高通量基因分型技术构建了129份橡胶树品种的特异DNA指纹图谱,5对引物共扩增出71条带,全部为多态性条带,引物平均PIC值达到0.69。基于129份品种的特异DNA指纹图谱,建立了每份品种的DNA指纹二维码。研究成果的获得为橡胶树品种鉴定提供了一种准确、稳定、高效的鉴定方法,也为橡胶树品种知识产权保护提供了可靠的技术支撑。Rubber tree is an important tropical economic crop.Rubber clones are mainly identified by morphological characters,which is difficult due to the minor morphological differences among them.In order to establish a stable,accurate,and efficient method for rubber clone identification,5(out of 426)pairs of SSR primers with clear and stable amplification profiles were selected as core primer sets.Based on these primer sets,high throughput genotyping of 129 rubber tree clones were performed.A total of 71 bands were detected,all of which were polymorphic.The average PIC of the primer pairs was 0.69.According to the polymorphic bands,specific DNA fingerprint of each clone was successfully constructed,and all of the specific DNA fingerprints were converted into quick response(QR)code.The results indicated that an effective method for rubber tree clone identification was constructed,which can provide sufficient technological supports for the protection of rubber tree clone intellectual property.

关 键 词：橡胶树 DNA指纹 分子标记 品种 

分 类 号：S794.1[农业科学—林木遗传育种]