黑龙江省苹果茎沟病毒的分布及遗传多样性分析  

Distribution and Genetic Diversity of Apple Stem Grooving Virus in Heilongjiang Province

作　　者：李鹏举 侯帅 宋鹏慧 杨光 房磊 段亚东 LI Peng-ju;HOU Hao;SONG Peng-hui;YANG Guang;FANG Lei;DUAN Ya-dong(Institute of Rural Revitalization Science and Technology,Heilongjiang Academy of Agricultural Sciences,Harbin 150028,China)

机构地区：[1]黑龙江省农业科学院乡村振兴科技研究所,黑龙江哈尔滨150028

出　　处：《黑龙江农业科学》2021年第4期59-62,共4页Heilongjiang Agricultural Sciences

基　　金：黑龙江省农业科学院青年基金项目(2020YYYF001);农业农村部园艺作物种质资源利用重点实验室开放基金(NYZS201907)。

摘　　要：为促进黑龙江省苹果茎沟病的预防,从而加强优质苹果无毒苗木的推广和产业升级,本试验通过对在黑龙江省采集到的60份疑似感苹果茎沟病毒(ASGV)的苹果样品进行RT-PCR特异性检测,明确了ASGV在黑龙江省6大苹果产区的发生分布范围。结果表明:牡丹江地区、松花江平原地区的苹果植株带毒率达到100%,且苹果品种金红的ASGV发生率最低。ASGV CP序列同源性以及遗传多样性分析表明ASGV-MDJ与ASGV-SHJ具有较近的亲缘关系,与ASGV-DN的亲缘关系较远。这3份分离物与印度和中国台湾、山东、陕西等地的ASGV核苷酸一致率达到98.56%以上。In order to promote the prevention of apple stem grooves disease in Heilongjiang Province,so as to strengthen the promotion of high quality apple non-toxic seedlings and industrial upgrading.In this study,60 suspected samples of apple stem grooving virus(ASGV)was specific detection by RT-PCR in Heilongjiang Province.We defined the ASGV occurrence and distribution of Heilongjiang six major apple-producing areas.The results showed that,the apple plants infected ASGV rate of Mudanjiang area and Songhua river plains reached 100 percent.And then,the ASGV lowest disease incidence was‘golden red’apple varieties.ASGV CP sequence homology and genetic diversity analysis showed that ASGV-MDJ and ASGV-SHJ had a closer relationship,and a father kinship with ASGV-DN.The ASGV nucleotide consistent rate of three isolates in this trial with India,Chinese Taiwan,Shandong Province and Shaanxi Province more than 98.56%.

关 键 词：苹果茎沟病毒 分布 序列同源性 遗传多样性 亲缘关系 

分 类 号：S43[农业科学—农业昆虫与害虫防治]