白藜芦醇改善高脂诱导老龄小鼠肥厚性肥胖作用的研究  

Effects of resveratrol on high-fat diet-induced hypertrophic obesity in elderly mice

作　　者：尹森[1] 毛敏 齐若梅[1] 冯璐 宫环[1] 鲍利[1] 薛芸 张明[1] 陈北冬[1] Yin Sen;Mao Min;Qi Ruomei;Feng Lu;Gong Huan;Bao Li;Xue Yun;Zhang Ming;Chen Beidong(The Key Laboratory of Geriatrics,Beijing Institute of Geriatrics,Beijing Hospital,National Center of Gerontology,National Health Commission,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]北京医院,国家老年医学中心,国家卫生健康委北京老年医学研究所,国家卫生健康委老年医学重点实验室,中国医学科学院老年医学研究院,100730

出　　处：《中华老年医学杂志》2021年第3期359-363,共5页Chinese Journal of Geriatrics

基　　金：国家自然科学基金(81270854,30900627);北京医院科技新星项目(BJ-2016-032)。

摘　　要：目的探讨白藜芦醇对老龄小鼠肥胖的影响及其可能的作用机制.方法分别将32周龄和48周龄小鼠随机分为3组:正常对照组进食普通饲料,每天灌胃1次0.3 ml生理盐水;高脂饮食处理组进食高脂饲料(含有21%脂肪和1.25%胆固醇),每天灌胃1次0.3 ml生理盐水;高脂饮食加白藜芦醇组进食高脂饲料,每天灌胃1次白藜芦醇(22.4 mg/kg,分散于0.3 ml生理盐水中).12周后称量各组小鼠体重,并进行脂肪组织取材及称重;采用酶联免疫吸附试验检测小鼠血浆瘦素浓度;通过实时荧光定量聚合酶链式反应方法检测小鼠肥厚性肥胖相关指标.结果高脂饮食加白藜芦醇组老龄小鼠体重及皮下脂肪含量分别为(34.43±3.23)g和(3.21±1.58)%,较高脂饮食组老龄小鼠(53.16±2.16)g和(4.86±0.64)%有明显下降(均P<0.01),并与普通饮食中年小鼠数据接近;与中年小鼠比较,白藜芦醇对高脂饮食老龄小鼠瘦素的抑制作用更为显著,高脂饮食加白藜芦醇组老龄小鼠血浆瘦素浓度(0.015±0.009)g/L,较高脂饮食组老龄小鼠(0.100±0.027)g/L明显下降,且低于普通饮食的老龄小鼠(F=19.85,P=0.001),与普通饮食中年小鼠相接近;白藜芦醇可以显著增加高脂饮食老龄小鼠皮下脂肪组织过氧化物酶体增殖物激活受体γ(PPARγ)和葡萄糖转运蛋白4(GLUT4)的表达,并降低肿瘤坏死因子-α(TNF-α)的表达(F=10.79、9.31、7.02,P=0.003、0.006,0.010).结论白藜芦醇可以显著改善老龄小鼠肥厚性肥胖,抑制瘦素分泌和上调PPARγ表达可能是其作用的关键机制.Objective To investigate the effects and potential mechanisms of resveratrol on obesity in elderly mice.Methods In this study,3 groups were randomly formed for 32-week-old mice and for 48-week-old mice.The normal diet group received regular chow and 0.3 ml saline by gavage once a day,the high-fat diet group received a high-fat diet(containing 21%fat and 1.25%cholesterol)and 0.3 ml saline once a day,and the high-fat diet plus resveratrol group received a high-fat diet and resveratrol(22.4 mg/kg,dispersed in 0.3 ml saline)by gavage once a day.After 12 weeks,body weight and adipose tissues were measured.Plasma leptin concentrations were determined by an enzyme-linked immunosorbent assay(ELISA),and values for hypertrophic obesity-related indexes of mice were obtained by quantitative real-time PCR.Results The body weight and the proportion of subcutaneous fat tissues were lower in the high-fat diet plus resveratrol group than in the high-fat diet group[(34.43±3.23)g vs.(53.16±2.16)g,(3.21±1.58)%vs.(4.86±0.64)%,P<0.01],and were similar to those in the normal diet group.Resveratrol had a more obvious inhibitory effect on leptin in elderly mice than in middle-aged mice.In elderly mice,the plasma leptin concentration was lower in the high-fat diet plus resveratrol group than in the high-fat diet group[(0.015±0.009)g/L vs.(0.100±0.027)g/L]and the normal diet group(F=19.85,P=0.001),and it was similar to that in the middle-aged mice on a normal diet.Resveratrol significantly increased the expression of peroxisome proliferator-activated receptor gamma(PPARγ)and glucose transporter 4(GLUT4)and reduced the expression of tumor necrosis factor-α(TNF-α)(F=10.79,9.31 and 7.02,P=0.003,0.006 and 0.010).Conclusions Resveratrol can significantly improve hypertrophic obesity in elderly mice,and the inhibition of leptin secretion and up-regulation of PPARγmay be the key mechanisms.

关 键 词：肥胖症 白藜芦醇 瘦素 

分 类 号：R285.5[医药卫生—中药学]