效应蛋白OhEF 2正调控拟南芥对橡胶树白粉菌的感病性  

Effector protein OhEF 2 enhances the susceptibility of Arabidopsis to Oidium heveae

作　　者：程度 戎伟[2] 梅双双[2] CHENG Du;RONG Wei;MEI Shuangshuang(College of Life Sciences and Pharmacy,Hainan University,Haikou,Hainan,570228;College of Plant Protection,Hainan University,Haikou,Hainan,570228)

机构地区：[1]海南大学生命科学与药学院,海口570228 [2]海南大学植物保护学院,海口570228

出　　处：《热带生物学报》2021年第1期75-82,共8页Journal of Tropical Biology

基　　金：国家自然科学基金(31760303,32060138)。

摘　　要：白粉菌通过在植物细胞内形成吸器,产生大量的效应蛋白,从而实现在寄主细胞内的侵染。前期有研究人员对Oidium heveae进行基因组和转录组测序分析,预测出133个潜在的效应蛋白。笔者克隆了其中的一个基因OhEP2(Oidium heveae Effector Protein 2),并构建了OhEF 2基因在拟南芥Col-0背景下的过表达转基因植株。通过接种发现,过量表达OhEF 2可以明显促进拟南芥对橡胶树白粉菌的感病性,但不能提高假单胞菌DC 3000的毒性功能,表明OhEF 2可能只在白粉菌侵染的过程中发挥作用。进一步研究发现,OhEF 2显著降低了橡胶树白粉菌在拟南芥上激发的胼胝体沉积和PR1(Pathogen-Related Gene1)基因表达,这为进一步研究效应蛋白OhEF 2在植物体内的毒性作用机理奠定了基础。Powdery mildew fungi complete its infection process on living host plants through the establishment of haustoria which secretes a plethora of effectors into plant cells.A previous study showed that 133 potential effectors of Oidium heveae have been predicted by genome and transcriptome sequencing.However,the biological function of these effectors are still unknown.In this study,an effector gene,named OhEF 2,was cloned and transformed into Arabidopsis,and the transgenic plants of OhEF 2 gene in Arabidopsis Col-0 background were constructed and inoculated with O.heveae.Inoculation assay showed that OhEF 2 obviously enhanced the susceptibility of Arabidopsis to O.heveae.However,OhEF 2 did not increase the virulence of Pseudomonas syringae DC 3000,suggesting that OhEF 2 probably plays a role only in the process of powdery mildew infection.Further study showed that OhEF 2 decreased the callose deposition and PR1 gene expression triggered by O.heveae in Arabidopsis,which lays a foundation for the future study of OhEF 2 virulence mechanisms in host plants.

关 键 词：橡胶树白粉菌 效应蛋白 OhEF 2 毒性机理 

分 类 号：Q943.2[生物学—植物学] S763.7[农业科学—森林保护学]