鳄鱼源气单胞菌的毒力基因与耐药特性分析  

Characteristics of Virulence Genes and Antibiotic Resistance of Aeromonas spp.Isolated from Siamese crocodile

作　　者：韩语 王昕 潘纪汶 杨诺 郭桂英[2] 李迁[3] 曾纪锋[4] 郑继平[1] HAN Yu;WANG Xin;Pan Jiwen;YANG Nuo;GUO Guiying;LI Qian;ZENG Jifeng;ZHENG Jiping(Institute of Life Sciences and Pharmacy,Hainan University,Haikou,Hainan 570228;College of Science,Hainan University,Haikou,Hainan 570228;Network and Technology Center,Hainan University,Haikou,Hainan 570228;College of Animal Science and Technology,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学生命科学与药学院,海口570228 [2]海南大学理学院,海口570228 [3]海南大学网络与技术中心,海口570228 [4]海南大学动物科技学院,海口570228

出　　处：《热带生物学报》2021年第1期96-102,共7页Journal of Tropical Biology

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC084);国家自然科学基金项目(32060131)。

摘　　要：对海南地区患病暹罗鳄(Crocodylus siamensis)体内分离出的10株气单胞菌(5株嗜水气单胞菌、3株达卡气单胞菌、1株简达气单胞菌和1株维氏气单胞菌)进行了毒力基因、耐药基因的检测以及抗生素药物敏感性试验。结果表明:气溶素、肠毒素、蛋白酶等基因检出率较高,其中,lip和ela 2种毒力基因检出率高达100%;喹诺酮类、四环素类、磺胺类、β-内酰胺类、氨基糖苷类耐药基因检出率大于50%;气单胞菌对喹诺酮类抗生素、四环素类抗生素、氨基糖苷类抗生素、氯霉素类和碳青霉烯类抗生素的敏感率较高,而对于β-内酰胺类抗生素、磺胺类抗生素和糖肽类抗生素耐药率较高。A total of 10 strains of Aeromonas spp.were isolated from sick Siamese crocodile in Hainan,including 5 strains of Aeromonas hydrophila,3 strains of Aeromonas dhakensis,1 strain of Aeromonas veronii and 1 strain of Aeromonas jandaei,and tested for virulence genes,antibiotic resistance genes,and antibiotic susceptibility.The results showed that aerosol genes,enterotoxin genes and protease genes had a higher detection rate,of which two virulence genes,lip and ela,had a detection rate of up to 100%and the genes resistant to quinolones,tetracyclines,sulfonamides,β-lactams,and aminoglycoside had a detection rate of higher than 50%.Antibiotic susceptibility testing showed that Aeromonas spp.were more sensitive to quinolone,tetracycline,aminoglycoside,chloramphenicol and carbapenem antibiotics,and more resistant toβ-lactam,sulfonamides and glycopeptide antibiotics.

关 键 词：鳄鱼 气单胞菌 毒力基因 耐药基因 药物敏感性 

分 类 号：S852.61[农业科学—基础兽医学]