表面活性剂对嗜盐嗜碱硫碱弧菌D301生成及利用硫颗粒的影响  

Effect of surfactants on the formation and metabolism of sulfur particles by Thialkalivibrio versutus D301

作　　者：刘志霞[1,2] 杨茂华 穆廷桢[2] 刘金龙 邢建民[2] LIU Zhixia;YANG Maohua;MU Tingzhen;LIU Jinlong;XING Jianmin(School of Biology and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018,China;Key Laboratory of Green Process and Engineering,Chinese Academy of Sciences,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]河北科技大学生物与工程学院,河北石家庄050018 [2]中国科学院过程工程研究所,中国科学院绿色过程与工程重点实验室,北京100190

出　　处：《微生物学通报》2021年第2期362-372,共11页Microbiology China

基　　金：国家自然科学基金(31872633,21878307,31800030);国家重点研发计划(2020YFA0906800)。

摘　　要：【背景】微生物脱硫是脱除气体中硫化氢的一种有效方法,其中,硫颗粒的生成与代谢是控制生物脱硫效率的关键,但目前相应的控制方法很少。【目的】研究不同种类表面活性剂对硫碱弧菌D301生成及利用硫颗粒的影响。【方法】通过摇床培养,利用X射线衍射、冷场发射扫描电镜、能谱分析及傅里叶红外光谱对硫颗粒进行表征。【结果】单质硫主要以S_(8)形式存在,吐温-80和十二烷基磺酸钠(Sodium Dodecyl Sulfate,SDS)的添加对硫颗粒的形态及生成量影响明显。对照组中生成的硫颗粒呈规则球形,光滑完整,其表面附着蛋白质等生物大分子;加入0.01 g/L吐温-80后,硫颗粒呈长杆状、颗粒增大、利用速率减慢;加入0.3g/L的SDS后,硫颗粒呈短棒状、生成量减少、利用速率加快,同时延缓了硫碱弧菌D301的衰亡。【结论】添加表面活性剂可以改变硫颗粒形态并且影响其利用,是一种调控硫颗粒生成和代谢的有效手段。[Background]Bio-desulfurization is an effective method of removing hydrogen sulfide from industrial gas,biogas,natural gas,etc.In the process of bio-desulfurization,the formation and metabolism of sulfur particles is the key steps that directly affect the efficiency of desulfurization.However,our understanding of the methods of regulating sulfur particles is still insufficient.[Objective]The effects of different surfactants on the formation and metabolism of sulfur particles by Thialkalivibrio versutus D301 were studied.[Methods]T.versutus D301 was cultured in erlenmeyer flask,and the sulfur-oxidizing bacterica and sulfur particles were characterized by X-ray diffraction(XRD),cold field emission scanning electron microscopy(SEM),energy dispersive system(EDS)and Fourier transform infrared spectroscopy(FTIR).[Results]The elemental sulfur in sulfur particle mainly exists in the form of S_(8).The addition of Tween-80 or SDS has a significantly effect on the morphology and production of sulfur particles.In the control experiment,the sulfur particles generated were regular spherical,smooth and intact,and biological macromolecules such as proteins cover to the surface of the sulfur particles.When 0.01 g/L Tween-80 is added,long rod-shaped and larger-sized sulfur particles are formed and the metabolic rate of elemental sulfur slows down.When 0.3 g/L SDS is added,the sulfur particles are in the form of short rods.Less sulfur particles are formed,and they are rapidly metabolized by T.versutus D301.[Conclusion]Regulating the morphology and metabolic rate of sulfur particles can be achieved by adding specific surfactants during bacterial cultivation.

关 键 词：生物脱硫 硫碱弧菌 硫颗粒 表面活性剂 

分 类 号：Q935[生物学—微生物学]