葡萄糖修饰罗丹明B的荧光探针的合成及其性质研究  

Synthesis and Application of Fluorescent Probes of Rhodamine B Modified with Glucose

作　　者：张钰绮 陈永虎 刘华丽 张斌 黄婉婷 刘翔元 董树国 ZHANG Yu-qi;CHEN Yong-hu;LIU Hua-li;ZHANG Bin;HUANG Wan-ting;LIU Xiang-yuan;DONG Shu-guo(College of Pharmacy,Jilin Medical University,Jilin 132013,China)

机构地区：[1]吉林医药学院药学院,吉林吉林132013

出　　处：《化学试剂》2021年第2期243-246,共4页Chemical Reagents

基　　金：吉林省大学生创新创业训练计划项目(201913706042)。

摘　　要：设计合成一种用于pH测定的荧光探针,以罗丹明B、1-O-丙炔基-2,3,4,6-四-O-乙酰基-β-D-葡萄糖苷等为原料,利用Click反应,合成葡萄糖修饰罗丹明B荧光探针,并利用核磁共振波谱对其结构进行表征。进一步研究了该探针的荧光性质,并利用MTT法评价了所制备荧光探针的细胞毒性。实验结果表明,弱酸性条件下,在578 nm处有强荧光发射峰。在pH 4.0~6.5范围内,荧光强度与pH呈线性关系,并具有良好的可逆性。细胞毒性试验中,当浓度≥40μmol/L时,细胞的生存能力开始降低,通过拟合计算半抑制浓度IC50为36.56μmol/L。所合成的荧光探针有望用于细胞微环境的pH检测。A fluorescent probe for pH measurement was designed and synthesized.Rhodamine B was modified with glucose by Click reaction using Rhodamine B,and 1,2,3,4,6-penta-O-acetyl-beta-D-glucose as raw materials.The structure of the fluorescent probe was confirmed by NMR.Its fluorescence properties was investigated,and cytotoxicity was evaluated by MTT.There was a strong fluorescence emission peak at 578 nm under weakly acidic conditions.The fluorescence intensity had an excellent linear relationship with pH 4.0~6.5.The fluorescent probe had a good reversibility.The results of cytotoxicity test showed that the viability of cells began to decrease when the concentration was more than 40 μmol/L.The semi-inhibitory concentration IC50 was 36.56 μmol/L by fitting calculation.The synthesized fluorescent probes are expected to be used for the pH detection of the cellular microenvironment.

关 键 词：罗丹明B 葡萄糖 荧光探针 CLICK反应 应用 

分 类 号：O657.3[理学—分析化学]