HPLC-MS鉴定黄芪水煎液中黄酮类成分  

HPLC-MS identification on flavonoids in Astragali Radix decoction

作　　者：谭雪霞 朱宏明 唐铖[2] 王雪[2] TAN Xue-xia;ZHU Hong-ming;TANG Cheng;WANG Xue(Department of Pharmacy,Sino-Singapore Eco-city Hospital of Tianjin Medical University,Tianjin 300467,China;School of Pharmacy,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学中新生态城医院药剂科,天津300467 [2]天津医科大学药学院,天津300070

出　　处：《现代药物与临床》2021年第2期231-235,共5页Drugs & Clinic

基　　金：天津市自然科学基金资助项目(18JCYBJC95100)。

摘　　要：目的建立黄芪水煎液中黄酮类成分HPLC-MS鉴定方法。方法黄芪水煎液采用C18固相萃取小柱获得黄酮类化学成分。采用HPLC-DAD-MS的方法对黄芪水煎液中黄酮类成分进行了定性研究,对比所建立化合物库的化学成分与实测的化学成分质荷比信息以及化合物的裂解规律确定各化学成分。结果液相色谱质谱联用技术可对黄芪水煎液所含黄酮类成分进行快速鉴别。根据相对分子质量信息确定了黄芪水煎液所含22个黄酮类化学成分的结构信息。结论高效液相-质谱联用技术可快速对黄芪水煎液所含黄酮类成分进行表征,为其药效物质基础研究提供新思路。Objective To establish an identification method for flavonoid chemical constituents in Astragali Radix decoction by HPLC-MS. Methods The samples were prepared by SPE C18 column. HPLC-DAD-MS method was used to identify the flavonoids in Astragali Radix decoction. The chemical constituents of the library were compared with the measured mass charge ratio of chemical composition in Astragali Radix decoction, and the chemical constituents was determined by the pyrolysis law of the compounds. Results HPLC-MS provided high analysis efficiency of chemical components in Astragali Radix decoction. Based on the mass information and MS fragmentation behaviors of the chemical components, 22 compounds in Astragali Radix decoction were identified. Conclusion HPLC-MS technology can quickly characterize the flavonoids in in Astragali Radix decoction, and provide a new idea for the study of its pharmacodynamic material basis.

关 键 词：黄芪水煎液 黄酮 高效液相色谱-质谱联用 鉴定 

分 类 号：R284.1[医药卫生—中药学]