多囊卵巢综合征患者肠道菌群和生化免疫分子特征  

Characteristics of intestinal microflora and biochemical immune molecules in patients with polycystic ovarian syndrome

作　　者：汪湾 尚潇潇 曾秋耀[3] 刘冬冬 李贝贝 张嘉琪 杨洪艳 黄译乐 胡薇 傅锦坚 徐建华 Wan Wang;Xiaoxiao Shang;Qiuyao Zeng;Dongdong Liu;Beibei Li;Jiaqi Zhang;Hongyan Yang;Yile Huang;Wei Hu;Jinjian Fu;Jianhua Xu(Department of Laboratory Science,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine(The Second Clinical Medical College of Guangzhou University of Chinese Medicine),Guangzhou 510120,Guangdong Province,China;Bioinformatics Group,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine(The Second Clinical Medical College of Guangzhou University of Chinese Medicine),Guangzhou 510120,Guangdong Province,China;Department of Clinical Laboratory Medicine,Sun Yat-sen University Cancer Center,State Key Laboratory of Oncology in South China,Collaborative Innovation Center for Cancer Medicine,Guangzhou 510060,Guangdong Province,China;Department of Gynecology,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine(The Second Clinical Medical College of Guangzhou University of Chinese Medicine),Guangdong Provincial Key Laboratory of clinical Research on Traditional Chinese Medicine Syndrome,Guangzhou 510120,Guangdong Province,China;Shunde Hospital of Guangzhou University of Chinese Medicine,Foshan 523888,Guangdong Province,China;Guangxi Liuzhou Maternal and Child Health Hospital,Liuzhou 545001,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广州中医药大学第二附属医院(广州中医药大学第二临床医学院)检验医学部,广东广州510120 [2]广州中医药大学第二附属医院(广州中医药大学第二临床医学院)生物信息分析中心,广东广州510120 [3]中山大学肿瘤防治中心检验科,华南肿瘤学国家重点实验室,癌症医学协同创新中心,广东广州510060 [4]广州中医药大学第二附属医院(广州中医药大学第二临床医学院)妇产科,广东省中医药综合症临床研究广东省重点实验室,广东广州510120 [5]广州中医药大学顺德医院,广东佛山523888 [6]广西柳州市妇幼保健院,广西柳州545001

出　　处：《微生物学报》2021年第2期452-468,共17页Acta Microbiologica Sinica

基　　金：广东省中医院科学技术研究重点项目(YN2016ZD03);广西卫生科研基金(Z20180023);柳州市科学研究与技术开发项目(2017BH20310)。

摘　　要：【目的】比较多囊卵巢综合征(polycysticovariansyndrome,PCOS)患者与健康对照间肠道菌群谱结构特征差异,并探讨多囊卵巢综合征患者差异菌群与生化免疫指标之间的联系。【方法】选取初诊多囊卵巢综合征患者23例和健康女性对照23例,每例患者于初诊时留取粪便标本和血液标本,粪便标本提取DNA进行宏基因组测序,血清用于检测性激素和炎症因子。通过生物信息分析比较多囊卵巢综合征患者与健康对照间肠道菌群谱结构特征差异,以及与相关指标的联系。【结果】发现多囊卵巢综合征患者与健康对照的肠道菌群在门、属、种水平均有明显差异。两组间差异菌种为4个,普通拟杆菌(Bacteroides vulgatus)和弗格森埃希菌(Escherichia fergusonii)在PCOS组中的相对丰度高于对照组,并且这两种菌与PCOS的发病机制密切相关。凸腹真杆菌(Eubacteriumventriosum)和Subdoligranulum unclassified菌的相对丰度低于健康对照组。其中普通拟杆菌、凸腹真杆菌和Subdoligranulum unclassified菌3个菌种彼此间存在相关性,差异菌种与生化免疫指标间也存在相关性。发现布鲁米球菌、棒状戈登菌、脱硫弧菌、凸腹真杆菌、斯特雷沃菌5个菌的组合在PCOS患者早期诊断中具有较好的诊断效能。基因分析发现,鸟苷核苷酸从头生物合成的超途径I基因通路在PCOS人群中富集。【结论】多囊卵巢综合征患者与对照组间的肠道菌群和生化免疫指标均具有差异,并具有一定相关性,为PCOS患者的综合性诊疗提供了方向。[Objective] To compare the structural characteristics of intestinal flora between patients with polycystic ovary syndrome and healthy controls, and to explore the relationship between differential flora and biochemical immune indicators in patients with polycystic ovary syndrome. [Methods] From March 2018 to May 2019, 23 patients with polycystic ovary syndrome and 23 healthy women who were treated at the gynecological clinic of The Second Affiliated Hospital of Guangzhou University of Chinese Medicine were selected. Each patient retained a stool specimen and a blood specimen at the initial diagnosis. Fecal specimens were extracted from DNA for metagenomic sequencing, and serum was used to detect sex hormones and inflammatory factors. Biological information analysis was used to compare the structural characteristics of intestinal flora between patients with polycystic ovary syndrome and healthy controls. [Results] The intestinal flora of patients with polycystic ovary syndrome and healthy controls were significantly different at the Phylum, Genus and Species levels. There are 4 species with a marked difference between the two groups. The relative abundance of Bacteroides vulgatus and Escherichia fergusonii in the polycystic ovary syndrome group is higher than that in the control group, and these two bacteria are closely related to the pathogenesis of polycystic ovary syndrome. While the relative abundance of Eubacterium ventriosum and Subdoligranulum unclassified is significantly lower than that of the healthy control group. Among them, Bacteroides vulgatus, Eubacterium ventriosum and Subdoligranulum unclassified are correlated with each other, and there are correlations between different species and biochemical immune indexes. It was found that the combined of five species of Ruminococcus bromii, Gordonibacter pamelaeae, Desulfovibrio piger, Eubacterium ventriosum, Prevotella stercorea had good efficacy in the early diagnosis of polycystic ovary syndrome patients. Genetic analysis found that PWY 7228 super-pathwa

关 键 词：多囊卵巢综合征 肠道菌群 生化 免疫 诊断 

分 类 号：R711.75[医药卫生—妇产科学]