显微共聚焦拉曼技术在细菌分类鉴定中的应用  

Application of micro confocal Raman technique in classification and identification of bacteria

作　　者：高鹏亚 苏英会 孙晖[3] 滕中秋[3] 吴长德[1] 史芸 袁洪福[4] 刘洋[2] 徐雪芳[3] Gao Pengya;Su Yinghui;Sun Hui;Teng Zhongqiu;Wu Changde;Shi Yun;Yuan Hongfu;Liu yang;Xu Xuefang(Shenyang Agricultural University,Shenyang 110866,Liaoning,China;Department of Food Science,College of Biochemical Engineering,Beijing Union University,Beijing 100023,China;State Key Laboratory of Infectious Disease Prevention and Control,Center for Disease Control and Prevention,Infectious Disease Prevention and Control Institute,Beijing 102206,China;Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]沈阳农业大学,辽宁沈阳110866 [2]北京联合大学,北京100023 [3]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206 [4]北京化工大学,北京100029

出　　处：《疾病监测》2021年第1期74-81,共8页Disease Surveillance

基　　金：国家重点研发计划(No.2018YFC1603800);2019年北京联合大学高水平人才培养交叉计划–实培计划毕业设计项目。

摘　　要：拉曼光谱可以用于识别和描述微生物的特征,作为当今微生物分类鉴定研究的热点。相比于传统的微生物分离培养技术和分子生物学鉴定技术,拉曼技术具有免培养、快速和高效等优点。随着光学领域拉曼技术和分子生物学的结合发展,使得微生物的鉴定变得更加高效快捷。拉曼显微光谱结合稳定同位素探针、荧光原位杂交和光镊技术,为研究生态系统中不可培养微生物的功能和生理提供了一种独立的方法。显微共聚焦拉曼技术在细菌分类鉴定中具有巨大的应用前景。本文主要阐述拉曼光谱技术的基础知识和显微共聚焦拉曼技术在细菌分类鉴定中的研究进展,并对该技术的优缺点进行总结和评价,旨在为拉曼光谱技术应用于微生物学的研究提供参考。Raman spectroscopy can be used to identify and describe the characteristics of microorganisms.Compared with the traditional microorganism isolation and culture methods and molecular biology identification technique,Raman technique has the advantages of non-culture,rapidness and high efficiency.With the combination of Raman technique and molecular biology in the field of optics,microbial identification has become more efficient and rapid.Raman microspectroscopy,combined with stable isotope probe,fluorescence in situ hybridization and optical tweezers,provides an independent method for studying the function and physiology of non-culpable microorganisms in ecosystems.The microconfocal Raman technique has a great application prospect in the identification and classification of bacteria.In this paper,the basic knowledge of Raman spectroscopy and the research progress of microconfocal Raman technique in classification and identification of bacteria are described,and the advantages and disadvantages of this technique are summarized and evaluated,in order to provide reference for the applied microbiology research of Raman technique.

关 键 词：显微共聚焦拉曼 表面增强拉曼散射 细菌分类鉴别 

分 类 号：R211[医药卫生—中医学] R372