Dispen-Seq: a single-microparticle dispenser based strategy towards flexible cell barcoding for single-cell RNA sequencing  

作　　者：Tian Tian Yingwen Chen Yunpeng Bi Xingrui Li MingXuan Gao Xuebing Zhang Weidong Ruan Jia Song Xing Xu Lingling Wu Zhi Zhu Yanling Song Chaoyong Yang 

机构地区：[1]State Key Laboratory for Physical Chemistry of Solid Surfaces,Key Laboratory for Chemical Biology of Fujian Province,The MOE Key Laboratory of Spectrochemical Analysis&Instrumentation,Department of Chemical Biology,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China [2]Institute of Molecular Medicine,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China [3]Chemistry and Biomedicine Innovation Center(ChemBIC),School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China

出　　处：《Science China Chemistry》2021年第4期650-659,共10页中国科学（化学英文版）

基　　金：supported by the National Key R&D Program of China(2018YFC1602900);the National Natural Science Foundation of China(21927806,21735004,222022409,21874089,21705024,21775128);the Program for Changjiang Scholars and Innovative Research Team in University(IRT13036)。

摘　　要：Single-cell RNA sequencing(sc RNA-seq)has become one of the most powerful tools to understand the heterogeneity of biological systems.While barcoding strategies have revolutionized the field of high-throughput sc RNA-seq,it is still challenging to achieve highly efficient,direct and universal cell barcoding with cost-effectiveness and minimal sample loss.Herein,a single micro-particle dispenser approach for rapid single barcode bead/cell manipulation and pairing,enabling highly efficient cell barcoding for sc RNA-seq(Dispen-Seq)was developed.Notably,Dispen-Seq provides a versatile platform which can enrich cell subgroups of interest while unlimited by input sample amounts,and can respond to changes in sample composition with high resolution and reproducibility.It is anticipated that Dispen-Seq will increase the scope of sc RNA-seq from academic research to practical applications.

关 键 词：single cell analysis MICROFLUIDICS BARCODING scRNA-seq 

分 类 号：Q811.4[生物学—生物工程]