甲酸与双甲脒对意大利蜜蜂的时间-剂量-致死率分析  

Time-dose-mortality of formic acid and amitraz on western honeybee,Apis mellifera ligustica

作　　者：苗春辉 杨娟 黄新球 荀利杰 胡宗文 MIAO Chunhui;YANG Juan;HUANG Xinqiu;XUN Lijie;HU Zongwen(Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences,Mengzi 661101,Yunnan Province,China)

机构地区：[1]云南省农业科学研究院蚕桑蜜蜂研究所,云南蒙自661101

出　　处：《农药学学报》2021年第1期131-138,共8页Chinese Journal of Pesticide Science

基　　金：云南蚕桑蜜蜂研究专项(2018CF07).

摘　　要：为研究甲酸和双甲脒对意大利蜜蜂的致死规律,采用摄入法测定了两种杀螨剂对意大利蜜蜂成年工蜂的致死效应,并通过时间-剂量-致死率(TDM)模型进行了模拟,以探明两种药剂对蜜蜂的致死动态及时间-剂量效应,旨在为田间合理应用这两种药剂提供理论指导。结果表明:通过Hosmer-Lemoshow拟合异质性检验,意大利蜜蜂对两种杀螨剂的敏感程度次序为双甲脒>甲酸;甲酸和双甲脒对意大利蜜蜂的致死率高峰分别出现在处理后60 h和42 h;在1和10μg/mL下,甲酸和双甲脒的LT50值分别为4.8、4.2 h和1.5、1.4 h,处理后66 h的LD50和LD90值分别为7.40×10^(−2)、2.99×10^(−2)和4.21×10^(-2)、2.60×10^(−2)μg/mL。研究表明,高浓度的甲酸和双甲脒对意大利蜜蜂工蜂具有较强的致死效应,采用时间-剂量-致死率模型模拟能很好地表征甲酸和双甲脒对蜜蜂的致死规律。In order to investigate the lethal effects of formic acid and amitraz on honeybee workers,Apis mellifera ligustica,the toxicity of formic acid and amitraz against western honeybee were measured using the bioassay method of insecticide stomach poisoning activity under laboratory condition.And they were also tested by time-does-mortality(TDM)model to confirm the dynamics and does-mortality of the lethal effects.Those data would provide theoretically guide in practical application in the field.The results showed that,by the Hosmer-Lemeshow test,the sensitivities of adult workers to the concentration change of the acaricides were formic acid>amitraz.The apex mortality rates of formic acid and amitraz to honeybee workers was determined at 60 h and 42 h respectively.The median lethal time(LT50)of formic acid and amitraz under the concentration of 1μg/mL was 4.8 h and 4.2 h,respectively.And that under 10μg/mL was 1.5 h and 1.4 h,respectively.The values of LD50 and LD90 at 60 h after the treatment was 7.40×10^(–2)μg/mL,2.99×10^(–2)μg/mL and 4.21×10^(–2)μg/mL,2.60×10^(–2)μg/mL,respectively.High concentration of the formic acid and amitraz has stronger lethal effect for honeybee workers and time-does-mortality model accurately reflected the process of insecticides for western honeybees.

关 键 词：甲酸 双甲脒 意大利蜜蜂 寄生螨 毒力 时间-剂量-致死率模型 

分 类 号：TQ454[化学工程—农药化工] S481.1[农业科学—农药学]