一种高效、准确测定油菜中维生素C含量的方法  

An efficient and accurate method for determination of vitamin C in rapeseed

作　　者：王超 匡列琼 潘昱颍 顿小玲[1] 王新发[1] WANG Chao;KUANG Lie-qiong;PAN Yu-ying;DUN Xiao-ling;WANG Xin-fa(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory Biology and Genetic Im-provement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Hubei University,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]湖北大学,湖北武汉430062

出　　处：《中国油料作物学报》2021年第2期346-352,共7页Chinese Journal of Oil Crop Sciences

基　　金：农业行业标准项目(高维生素C油菜薹);中国富硒产业研究院富硒专项科技计划(2018FXZX02-10)。

摘　　要：为准确测定出油菜中维生素C的含量,在国标基础上,建立了一种高效、准确测定新鲜油菜维生素C含量的方法。结果表明:维生素C在测定范围内峰面积与标准液浓度线性关系良好,相关系数为0.9999。油菜维生素C含量测定的回收率为97.97%~101.40%,相对标准偏差RSD仅为1.84%,说明用该方法重现性良好,具有快速准确、稳定可靠等特点。贮藏温度越低,维生素C降解的速度越慢,用液氮贮藏样品更有利于维生素C的保存和精准鉴定;利用液氮冷冻并研磨样品更有利于样品中维生素C的提取,测得油菜中维生素C含量与仅均质化处理相比平均增加了约16%;液氮中贮藏的样品在不同时间下测定其维生素C含量并无显著变化,测得维生素C含量的RSD均小于2%。利用此方法可以测定较长时间保存样品中的维生素C,可满足高通量、高准确率的要求,有利于油菜的菜用营养和育种研究。Based on the national standard,an efficient and accurate method was used to determinate vitamin C in fresh samples of oilseed rape.Results showed well linear relationship between peak area and concentration of standard vitamin C liquid,with the correlation coefficient of 0.9999.The recovery rate of vitamin C in rapeseed was97.97%to 101.40%.The relative standard deviation was 1.84%only,indicating that this method had good reproducibility for rapeseed vitamin C determination.Results also showed that the lower temperature during storage,the slower degradation rate of vitamin C.Liquid nitrogen storage was more beneficial to preservation and accurate identification.The use of liquid nitrogen for sample grind was more beneficial to vitamin C extraction from the samples.Compared with no grinding,vitamin C content was increased by about 16%.With the extension of storage time,vitamin C content in samples stored in liquid nitrogen did not change significantly,and the relative standard deviation of vitamin C was less than 2%.Thus the technique could be used to study the variation of vitamin C in large population with high efficiency,accuracy and repeatable features.It was expected to be bencticial to the regetable nutrition and breeding research of rapeseed.

关 键 词：油菜 液氮 高效液相色谱法 维生素C 

分 类 号：S565.4[农业科学—作物学]