4种淫羊藿黄酮苷类主要成分体外药理活性比较  

Comparison of pharmacological activities in vitro of four flavonoid glycosides from Epimedii Folium

作　　者：史阔豪 张光财 武康雄 王彬 万闽歌 李菡 SHI Kuo-hao;ZHANG Guang-cai;WU Kang-xiong;WANG Bin;Wan Min-ge;LI Han(School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi′an 710021, China;School of Medicine and Pharmacy, Shaanxi University of Business & Commerce, Xi′an 712046, China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]陕西商贸学院医药学院,陕西西安712046

出　　处：《陕西科技大学学报》2021年第2期56-61,共6页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(81803784);中国博士后科学基金面上项目(211956);陕西省科技厅自然科学基金项目(2019JQ-779);陕西科技大学博士科研启动基金项目(2016BJ-57);陕西科技大学大学生创新创业训练计划项目(2019007)。

摘　　要：为了比较淫羊藿黄酮苷类4种主要成分朝藿定A,朝藿定B,朝藿定C和淫羊藿苷的抗菌、抗氧化和抗肿瘤活性,采用滤纸片扩散法和双倍稀释法测定四者对大肠杆菌和金黄色葡萄球菌的抑菌效果和最小抑菌浓度(MIC),对其抗菌活性进行评价.采用紫外可见分光光度计测定其清除自由基能力(DPPH和ABTS),对其抗氧化活性进行评价.采用CCK-8法测定其对BEL-7402细胞增殖和活性氧(ROS)含量的影响,对其抗肿瘤活性进行评价.研究发现,朝藿定A和淫羊藿苷对大肠杆菌和金黄色葡萄球菌表现出明显的抑菌活性,朝藿定B和朝藿定C均无明显抑菌活性.抗氧化活性:淫羊藿苷>朝霍定C>朝霍定A>朝霍定B,表明淫羊藿苷结构C3位鼠李糖基上进行糖基取代会减弱其抗氧化性.朝藿定A、B、C和淫羊藿苷均能抑制BEL7402细胞的增殖,相同浓度比较,朝藿定C和淫羊藿苷有更加明显的抑制作用,且抑制作用呈浓度依赖性.进一步检测对ROS含量影响,表明朝霍定C可能是通过调节细胞内ROS的含量诱导BEL-7402细胞死亡.项目采用生物活性为导向筛选,为淫羊藿黄酮苷化合物药用价值的利用提供依据.In order to investigate the antibacterial,anti-oxidant and anti-tumor activities of four compounds of Epimedii flavonoids glycosides(epimedin A,epimedin B,epimedin C and icariin),the antibacterial activity was determined according to the diameter of inhibition zone by the filter paper diffusion method and minimum inhibitory concentrations by double dilution method.UV-visible spectrophotometer was used to measure its free radical scavenging ability(DPPH and ABTS),and its antioxidant activity was evaluated.CCK-8 method was used to determine its effect on the proliferation of BEL-7402 cells,2′,7′-dichlorofluorescin diacetate(DCF-DA)staining and fluorescence microplate were applied to detect level of ROS in cells,and its antitumor activity was evaluated.The compound Epimedin A and icariin have obvious antibacterial activity Escherichia coli and Staphylococcus aureus,while epimedin B and epimedin C have no obvious antibacterial activity..The antioxidant activity:icariin>epimedin C>epimedin A>epimedin B,indicating that the glycosyl substitution on the rhamnosyl group at position C3 of the icariin structure will weaken its resistance oxidative.Four compounds of Epimedii flavonoids glycosides can inhibit the proliferation of BEL7402 cells.Compared with the same concentration,epimedin C and icariin have more obvious inhibitory effects,and the inhibitory effect is concentration-dependent.Further testing of the effect on ROS content indicated that epimedin C may induce BEL-7402 cell death by increasing the intracellular ROS content.This project adopts biological activity-oriented screening to provide a basis for the utilization of the medicinal value of Epimedium flavonoid glycoside compounds.

关 键 词：淫羊藿黄酮苷 朝霍定 抗菌 抗氧化 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学]