胭脂鱼寄生单殖吸虫形态特征及其系统发育分析  

Supplemental description and phylogeny of Pseudomurraytrema asiaticus from Chinese sucker fish, Myxocyprinus asiaticus

作　　者：翟凯旋 习丙文[1,2] 陈凯[2] 潘良坤[2] 朱新鹏 ZHAI Kaixuan;XI Bingwen;CHEN Kai;PAN Liangkun;ZHU Xinpeng(Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;Key Laboratory of Freshwater Fisheries and Gerplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Jiangsu Zhongyang Group Co.,Ltd,Nantong 226600,China)

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [3]江苏中洋集团长江珍稀鱼类繁养殖基地,江苏南通226600

出　　处：《中国水产科学》2021年第3期364-370,共7页Journal of Fishery Sciences of China

基　　金：农业农村部大宗淡水鱼产业技术体系项目(CARS-45);中国水产科学研究院淡水渔业研究中心基本科研业务费项目(2019JBFZ08)。

摘　　要：为准确鉴定严重危害保育胭脂鱼(Myxocyprinus asiaticus)的单殖吸虫(Monogenea)种类,本研究采用形态特征和LSU rDNA序列分析相结合的手段对所发现样品进行准确鉴定和描述。在养殖基地胭脂鱼鳃部所检查到的单殖吸虫形态特征如下:虫体呈长带状,前端具有口吸盘;阴道细长,交配管呈钩状,具有中央刺,卵茄形;具2对中央大钩,固着器与虫体分界明显,覆盖有指向前方的几丁质棘。LSU rDNA序列分析表明该单殖吸虫与美国亚口鱼(Catostomus ardens)寄生的一种伪缪丽虫(Pseudomurraytrema sp.)相似率最高(91.7%,AF382059);系统发育分析表明其与鳞盘虫科类群亲缘关系较近并位于分支基部。综合形态和分子数据,本研究所发现寄生于胭脂鱼鳃部的单殖吸虫被鉴定为亚洲伪缪丽虫。本文补充了亚洲伪缪丽虫的形态学特征和LSU rDNA序列数据,为进一步研究胭脂鱼寄生亚洲伪缪丽虫的疾病防控提供基础资料。This study aimed to identify the monogean flatworms that are seriously affecting the conservation of populations of Chinese sucker fish, Myxocyprinus asiaticus. To do so, we used the morphological characteristics and LSU rDNA sequence to identify and describe the worms detected from Chinese sucker fish. The worms were identified as Pseudomurraytrema asiaticus and had the following characteristics: the body was long-banded, the dorsal abdomen was flat, and the front end had an oral sucker. The vagina was slender, the mating tube was hook-shaped with central thorns, and the egg-shaped worms had two pairs of central large hooks. The boundary between the retainer and the insect body was clearly discernable and covered with chitin spines pointing forward. LSUrDNA sequence analysis showed that the obtained sequence had the highest similarity with Pseudomurraytema sp.(91.7%, AF382059) extracted from Utah sucker fish, Catostomus ardens, in the USA. Phylogenetic analysis revealed that the worms in this study were closely related to the family Diplectanidae and located at the base of its branches. In this study, we further describe the morphological characteristics and LSU rDNA sequence data of P. asiaticus. We believe the results of our study provide basic data for further studies on the biology and disease control for M. cochinensis.

关 键 词：胭脂鱼 单殖吸虫 伪缪丽虫 形态特征 系统发育 

分 类 号：S941[农业科学—水产养殖]