核盘菌对大豆致病力差异与抗氧化酶系统酶活性的关系  

The Relationship between the Pathogenicity of S. sclerotiorum to Soybean and the Enzyme Activity of Antioxidant Enzyme System

作　　者：李易初[1] 石凤梅[1] 马立功[1] 刘佳[1] 孟庆林[1] LI Yi-chu;SHI Feng-mei;MA Li-gong(Plant Protection Institute,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086)

机构地区：[1]黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨150086

出　　处：《安徽农业科学》2021年第7期142-145,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省农业科学院院基金项目(2018YYYF019);国家特色油料产业技术体系(CARS-14)。

摘　　要：采集黑龙江省大豆菌核病样分离得到15个菌株,选用大豆离体叶片接种法鉴定菌株致病力强弱,测定致病力差异菌株体内POD、SOD、CAT活性。结果发现,黑龙江地区大豆菌核病菌致病力分化明显,菌株致病力分化与来源地无明显相关性;致病力差异菌株体内POD、SOD、CAT活性存在差异;60%供试菌株体内SOD、POD和CAT活性与致病力呈正相关,菌株致病力强则菌株体内相应的酶活性强;菌株CAT活性与其致病力相关性不明显,需进一步试验验证。Fifteen strains were isolated from the samples of Sclerotinia sclerotiorum on soybean in Heilongjiang Province,and the pathogenicity of the strains were determined by inoculating the leaves in vitro,and the enzymes activity of POD,SOD and CAT in the strains with different pathogenicity were determined.It showed that the pathogenicity of S.sclerotiorum on soybean in Heilongjiang was differentiated obviously,and there was no obvious correlation between the pathogenicity and the collection places.The enzyme activities of POD,SOD and CAT were different in 15 strains.The enzyme activities of POD,SOD and CAT in strains were correlated with pathogenicity of the strains,and the enzyme activities of POD,SOD and CAT in the 60%strains of the test were bigger which strains with stronger pathogenicity.There was no significant correlation between the activity of CAT in strains and its pathogenicity.

关 键 词：大豆 核盘菌 致病力 酶活性 POD SOD CAT 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]