温度和盐度对中间球海胆(Strongylocentrotus intermedius)早期胚胎发育率的联合效应  

Interactive effects of temperature and salinity on embryonic development rate of Strongylocentrotus intermedius

作　　者：甄昊 吴洋磊 王荦[1] 裴泓霖 谭八梅 丁君[1] 常亚青[1] ZHEN Hao;WU Yang-lei;WANG Luo;PEI Hong-lin;TAN Ba-mei;DING Jun;CHANG Ya-qing(Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,Liaoning,China)

机构地区：[1]大连海洋大学,农业农村部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《生态学杂志》2021年第2期460-469,共10页Chinese Journal of Ecology

基　　金：国家重点研发计划项目(2018YFD0901604);辽宁省高等学校创新团队及创新人才支持计划(LT2019003);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402)资助。

摘　　要：海洋生物种群繁衍目前面临着严峻的环境压力。胚胎期作为生命发育的起始阶段,极易受环境因素的影响,温度和盐度则是影响生物生长发育的两大关键要素。为探明中间球海胆早期胚胎发育对温度和盐度的耐受性及其最适温盐条件,采用中心复合设计(CCD)和响应曲面法(RSM),开展温度(12~26℃)和盐度(22~34)对中间球海胆胚胎早期发育的联合效应研究,旨在建立温度和盐度对中间球海胆胚胎受精率、上浮率和变态率的定量关系模型,并通过统计优化方法得到温度和盐度的最佳组合。结果表明:过高或过低的温度和盐度均不利于海胆早期胚胎发育率,温度对中间球海胆早期胚胎发育的影响大于盐度,且温度和盐度之间存在一定的拮抗作用;温度和盐度的一次效应、温度的二次效应均极显著影响中间球海胆胚胎受精率、上浮率和变态率(P<0.01);温度和盐度的互作效应显著影响中间球海胆胚胎受精率和变态率(P<0.05),对上浮率的影响不显著(P>0.05);盐度的二次效应极显著影响中间球海胆胚胎受精率(P<0.01),对上浮率和变态率的影响不显著(P>0.05);实验建立的受精率、上浮率和变态率模型方程决定系数分别为0.9736、0.9946和0.9925,表明该模型建立有效并可用于预测中间球海胆胚胎受精率、上浮率和变态率。模型优化和验证试验得出,温度17.71℃和盐度31.85时,受精率、上浮率和变态率达最大值,满意度为0.969。研究结果可为中间球海胆人工繁育提供科学依据。The growth of marine organisms is currently challenged by multiple environmental stressors.The early life stages of sea urchin Strongylocentrotus intermedius are sensitive to environmental changes.Temperature and salinity are important environmental factors affecting the growth and development of marine animals.To understand the temperature and salinity tolerance of the early embryonic development of sea urchin and its optimal environmental conditions,we used the central composite design(CCD)and response surface methodology(RSM)to investigate the interactive effects of temperature(12-26℃)and salinity(22-34)on the early development of the sea urchin embryo.The quantitative relationship models between temperature,salinity and the fertilization rate,floating rate and metamorphosis rate of sea urchin embryos were established,and the best combination of temperature and salinity was obtained through statistical optimization methods.The results showed that both high and low temperature and salinity were not conducive to the early embryonic development rate of sea urchins.The effect of temperature on the early embryonic development of S intermedius was greater than that of salinity,with certain antagonism between temperature and salinity.The primary effects of temperature and salinity,and the secondary effects of temperature significantly affected the fertilization rate,floating rate,and metamorphosis rate of the S.intermedius embryos(P<0.01).The interactive effects of temperature and salinity significantly affected the embryo fertilization rate and metamorphosis rate of S.intermedius(P<0.05),but did not affect the floating rate.The secondary effect of salinity significantly(P<0.01)affected the fertilization rate of the S.intermedius embryos,but did not affect the floating rate and metamorphic rate.The determination coefficients of the fertilization rate,floating rate and metamorphosis rate were 0.9736,0.9946,and 0.9925,respectively,indicating that the models were effective and could be used to predict the fertilizatio

关 键 词：中间球海胆 胚胎发育率 温度 盐度 联合效应 

分 类 号：S917.4[农业科学—水产科学]