反相高效液相色谱法同时测定利肝宁片中对羟基苯乙酮和绿原酸的含量  

Content Determination of Hydroxyacetophenone and Chlorogenic Acid in Liganning Tablets by RP-HPLC

作　　者：覃芳[1] 李广胜[1] 郭兴辉[2] 胡文文[1] 刘会丽[1] QIN Fang;LI Guangsheng;GUO Xinghui;HU Wenwen;LIU Huli(The Third Affliated Hospital of Henan University of Chinese Medicine,Zhengzhou Henan China 450008;Food and Drug Inspection and Testing Institution of Henan Province,Zhengzhou Henan China 450003)

机构地区：[1]河南中医药大学第三附属医院,河南郑州450008 [2]河南省食品药品检验所,河南郑州450003

出　　处：《中医学报》2021年第4期833-837,共5页Acta Chinese Medicine

基　　金：河南省中医药科学研究专项课题项目(2017ZY 2027)。

摘　　要：目的:建立利肝宁片中对羟基苯乙酮和绿原酸的含量测定方法,为利肝宁片的质量控制提供依据。方法:采用紫外双波长同时测定的反相高效液相色谱法。色谱柱:安捷伦ZORBAX Eclipse XDB C18;流动相:(体积分数)0.05%磷酸溶液-乙腈(9010);流速:1.0 mL·min^(-1);柱温:35℃;检测波长:275 nm(对羟基苯乙酮)和327nm(绿原酸)。结果:在选定的色谱条件下,对羟基苯乙酮峰和绿原酸峰与其他峰分离度良好。对羟基苯乙酮浓度在2.545~20.36 mg·L^(-1)范围内与峰面积有良好的线性关系(r=0.9997);平均回收率(n=6)为98.97%,RSD=1.72%。绿原酸浓度在10.31~82.47 mg·L^(-1)范围内,与峰面积有良好的线性关系(r=0.9998);平均回收率(n=6)为98.05%,RSD=1.04%。结论:该方法专属性强、灵敏度高,可用于利肝宁片中对羟基苯乙酮和绿原酸的含量测定。Objective:To establish a content determination method for hydroxyacetophenone and chlorogenic acid in Liganning Tablets and provide the basis of the quality control.Methods:RP-HPLC measurement using two different UV wavelengths simultaneously was adopted.Column ZORBAX Eclipse XDB C18,mobile phase was Phosphoric acid 0.05%Acetonitrile(90:10),the flow rate was 1.0 mL·min^(-1),the column temperature 35℃,the detection wavelength was 275 nm for hydroxyacetophenone and 327 nm for chlorogenic acid.Results:The hydroxyacetophenone and chlorogenic acid can completely separate from the other component in the selected chromatographic conditions.Of hydroxyacetophenone:Good linear relationship was presented in the range of 2.545-20.36 mg·L^(-1)(r=0.9997).The average recovery rate was 98.97%,RSD=1.72%,(n=6).Of chlorogenic acid:A good linear relationship was found in the range of 10.31-82.47 mg·L^(-1)(r=0.9997).The average recovery was 98.05%,RSD=1.04%(n=6).Conclusion:The method is specific,sensitive,and can be used in the content determination of hydroxyacetophenone and chlorogenic acid content in Liganning tablets.

关 键 词：高效液相色谱法 利肝宁片 对羟基苯乙酮 绿原酸 

分 类 号：R284.2[医药卫生—中药学]