利用BSA-Seq方法鉴定谷丰B抗稻瘟病基因  

BSA-Seq Identification of Blast-resistance Genes in Gufeng B Rice

作　　者：陈子强[1] 陈松彪 郭新睿 颜静宛[1] 田大刚[1] 李刚[1] 王锋[1] CHEN Ziqiang;CHEN Songbiao;GUO Xinrui;YAN Jingwan;TIAN Dagang;LI Gang;WANG Feng(Fujian Key Laboratory of Genetic Engineering for Agriculture/Biotechnology Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Marine and Agricultural Biotechnology Laboratory,Institute of Oceanography,Minjiang University,Fuzhou,Fujian 350108,China)

机构地区：[1]福建省农业遗传工程重点实验室/福建省农业科学院生物技术研究所,福建福州350003 [2]闽江学院海洋研究所海洋与农业生物技术实验室,福建福州350108

出　　处：《福建农业学报》2021年第1期36-40,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划公益类专项(2018R1019-9);福建省自然科学基金项目(2017J01054)。

摘　　要：【目的】挖掘和鉴定谷丰B稻瘟病抗性基因,了解谷丰B稻瘟病抗性遗传模式。【方法】以谷丰B和日本晴杂交获得F1和F2代遗传群体,接种稻瘟菌不同生理小种并分析抗病遗传模式;在F2群体中挑选极端抗/感单株构建DNA混合池,利用群体分离分析法(BSA)定位关联区域。【结果】谷丰B对KJ201、RB22、CHNOS、RB6、2Y838-1、501-3和IR16-1等菌株均表现高抗性,表明谷丰B基因组可能携带了广谱高抗稻瘟病基因。谷丰B和日本晴杂交,F1群体表现抗501-3和IR16-1,F2群体的抗病/感病分离比不符合3∶1,推测谷丰B基因组存在多个位点影响稻瘟病抗性。对F2群体的极端抗病、感病混合池及亲本DNA进行全基因组测序,鉴定了1756964个单核苷酸多态性(SNPs)标记。分析子代△SNP-index,定位到2个与抗病性显著关联区间,分别为Chr.6:10082-11397 kb和Chr.11:120-266 kb。其中,6号染色体的关联区间与Pi2/9抗病位点等位,区间内含有4006个SNPs和623个插入缺失(InDels)标记;11号染色体的关联区间含有752个SNPs和195个InDels标记。【结论】谷丰B对强致病力501-3菌株抗性可能是由第6号和11号染色体上的基因共同控制。研究结果为谷丰B抗性基因的精细定位及基因克隆奠定了基础,并为水稻抗稻瘟病分子标记辅助选择提供标记资源。【Objective】The rice cultivar Gufeng B confers strong,broad-spectrum,durable resistance against various rice blast isolates.The present study was aim to identify and map the blast resistance gene(s)in Gufeng B.【Method】The F1 and F2 population were obtained by crossing Gufeng B and Nipponbare,and the genetic model of blast resistance was analyzed after inoculating with 7 strains of Magnaporthe grisea.Subsequently,F2 population was used to construct a resistant pool and a sensitive pool respectively,and to map the associated loci via the method of bulked segregation analysis.【Result】Gufeng B exhibited high resistance to all of the tested strains,such as KJ201,RB22,CHNOS,RB6,2Y838-1,501-3 and IR16-1,suggesting that Gufeng B may carry the broad-spectrum and high resistance genes.The F1 progenies from the cross between Gufeng B and Nipponbare conferred resistance against the strains 501-3 and IR16-1,and the segregation ratio of resistance and susceptibility among F2 progenies does not fit 3:1,assuming that the resistance against the strains 501-3 and IR16-1 were controlled by multiple locus in Gufeng B.Whole genome re-sequencing of the two parental lines Gufeng B and Nipponbare identified 1,756,964 SNPs.Calculation results of△SNP-index showed that there were two candidate loci conferring resistance to rice blast disease,which were located at Chr.6:10,082-11,397Kb,corresponding to the Pi2/9 locus,and Chr.11:120-266Kb.4006 SNPs and 623 InDels markers were searched within the interval of Chromosome 6,752 SNPs and 195 InDels within the corresponding region of Chromosome 11,respectively.【Conclusion】The resistance of Gufeng B to 501-3 strain may be controlled by two resistance genes on chromosomes 6 and 11.Our results laid the foundation for finely mapping and cloning the resistance genes in Gufeng B,and provided marker resources for molecular marker-assisted selection.

关 键 词：水稻 稻瘟病 抗性基因 谷丰B BSA 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]